Detailed information of TA system TA196901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1479434..1480017
• Replicon: chromosome
• Accession: NZ_CP071771
• Organism: Acinetobacter towneri strain GX7

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J4G46_RS06920
• Protein ID: WP_096900853.1
• Coordinates: 1479682..1480017 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: N9CN57
• Locus tag: J4G46_RS06915
• Protein ID: WP_004973383.1
• Coordinates: 1479434..1479682 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G46_RS06895 (J4G46_06895)	1474637..1475524	-	888	WP_179989497.1	sulfite exporter TauE/SafE family protein	-
J4G46_RS06900 (J4G46_06900)	1475751..1476926	+	1176	WP_179989495.1	lipid-A-disaccharide synthase	-
J4G46_RS06905 (J4G46_06905)	1476932..1477885	-	954	WP_208011678.1	AraC family transcriptional regulator	-
J4G46_RS06910 (J4G46_06910)	1478052..1479257	+	1206	WP_004973378.1	alkane 1-monooxygenase	-
J4G46_RS06915 (J4G46_06915)	1479434..1479682	+	249	WP_004973383.1	hypothetical protein	Antitoxin
J4G46_RS06920 (J4G46_06920)	1479682..1480017	+	336	WP_096900853.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J4G46_RS06925 (J4G46_06925)	1480107..1481306	-	1200	WP_154320715.1	acyl-CoA dehydrogenase family protein	-
J4G46_RS06930 (J4G46_06930)	1481358..1482668	-	1311	WP_004973388.1	acyl-CoA dehydrogenase family protein	-
J4G46_RS06935 (J4G46_06935)	1482938..1484311	-	1374	WP_004973390.1	AarF/ABC1/UbiB kinase family protein	-
J4G46_RS06940 (J4G46_06940)	1484455..1484760	-	306	WP_154320717.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1470144..1480017	9873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12262.07 Da
• Isoelectric Point: 6.2162

>T196901 WP_096900853.1 NZ_CP071771:1479682-1480017 [Acinetobacter towneri]
MNQGDIYLISLDPTLGHEQQGKRPVFVISPKSFNLITKMPIVLPITSGGNFARNMGFAVELIGGKTTGIVRCDQSRALDF
EARHARFLESSHADVLDEVLARYIAIFSPED

Nucleotide

• Length: 336 bp

>T196901 NZ_CP095510:2243777-2244028 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8866.38 Da
• Isoelectric Point: 7.3181

>AT196901 WP_004973383.1 NZ_CP071771:1479434-1479682 [Acinetobacter towneri]
MIHTNLRKVGGSVMLAVPPTLLKSLGLDAGKEVSLSIVNGKLVVEPKAKPSYKLADLLQEHQALALEQDAWHDVKAVGRE
EI

Nucleotide

• Length: 249 bp

>AT196901 NZ_CP095510:2244025-2244279 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: N9CN57