Detailed information of TA system TA196900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1138549..1140278
• Replicon: chromosome
• Accession: NZ_CP071771
• Organism: Acinetobacter towneri strain GX7

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: N9CQZ9
• Locus tag: J4G46_RS05370
• Protein ID: WP_004972057.1
• Coordinates: 1138549..1139199 (+)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: N9BAP7
• Locus tag: J4G46_RS05375
• Protein ID: WP_004972055.1
• Coordinates: 1139199..1140278 (+)
• Length: 360 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G46_RS05360 (J4G46_05360)	1134979..1135203	-	225	WP_180168591.1	AlpA family phage regulatory protein	-
J4G46_RS05365 (J4G46_05365)	1135654..1137483	-	1830	WP_208012308.1	hypothetical protein	-
J4G46_RS05370 (J4G46_05370)	1138549..1139199	+	651	WP_004972057.1	DUF4433 domain-containing protein	Toxin
J4G46_RS05375 (J4G46_05375)	1139199..1140278	+	1080	WP_004972055.1	macro domain-containing protein	Antitoxin
J4G46_RS05380 (J4G46_05380)	1140296..1141579	-	1284	WP_180186672.1	site-specific integrase	-
J4G46_RS05385 (J4G46_05385)	1141882..1142832	-	951	WP_208012309.1	M15 family metallopeptidase	-
J4G46_RS05390 (J4G46_05390)	1142836..1143402	-	567	WP_004972690.1	hypothetical protein	-
J4G46_RS05395 (J4G46_05395)	1143413..1144213	-	801	WP_208012310.1	type IVB secretion system protein IcmH/DotU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (16-216)

Antitoxin

• Macro domain (18-128)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25248.85 Da
• Isoelectric Point: 7.5076

>T196900 WP_004972057.1 NZ_CP071771:1138549-1139199 [Acinetobacter towneri]
VRTFHDVLNPEKALIWRIVHRDNIPWILANGLHCSNSSKLNEHWVPIGSSELIEKRQQHRVPVGTEGVLGDYIPFYFTPF
SPMLLNIKSGRGGVRQRANDEIVILVSSLHDLKKQQIPFVFTNSHAYYQWTEFYTDLSDLDKIDWSILQTRDFKRDMDDP
AKFERYQAEALIYQHCPTAALLGMLCYSEQVKSQLEYWLQQAKINIPVHARRGVYF

Nucleotide

• Length: 651 bp

>T196900 NZ_CP095510:1250686-1251093 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 360 a.a.
• Molecular weight: 40323.50 Da
• Isoelectric Point: 6.5001

>AT196900 WP_004972055.1 NZ_CP071771:1139199-1140278 [Acinetobacter towneri]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPKNMAAYSQACKAGQVVTGQMFVTETDELIGARWIINFPTK
QHWRDKSKIEWIDTGLQALKAFIIDHQVKSIAIPPLGAGNGGLSWSVVKPRIEAALSELDGVEIIIFEPTSQYQNVAKKT
GVTQLTPARAMIAELVRRYWVLGIECSLLEIQKLAWFLQRAIEKQKLSNELKLNFQANFYGPYAVNLGHLLNALDGSYLK
SDKRIPDCDPLDVIWFNDQQKQNVQTYLLTEAKGYMAAVDEAATLIEGFESPFGMELLATVDWLIHKEGCQANVDAIQQG
LARWPAGERWAQRKLDLFKPEHIQYALDKLNHFEPTQTA

Nucleotide

• Length: 1080 bp

>AT196900 NZ_CP095510:1250439-1250705 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: N9CQZ9

Antitoxin

• AlphaFold DB: N9BAP7