Detailed information of TA system TA196895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1371219..1371802
• Replicon: chromosome
• Accession: NZ_CP071770
• Organism: Acinetobacter towneri strain GX5

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J4G45_RS07460
• Protein ID: WP_096900853.1
• Coordinates: 1371467..1371802 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: N9CN57
• Locus tag: J4G45_RS07455
• Protein ID: WP_004973383.1
• Coordinates: 1371219..1371467 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G45_RS07435 (J4G45_07440)	1366422..1367309	-	888	WP_004973373.1	sulfite exporter TauE/SafE family protein	-
J4G45_RS07440 (J4G45_07445)	1367536..1368711	+	1176	WP_004973375.1	lipid-A-disaccharide synthase	-
J4G45_RS07445 (J4G45_07450)	1368717..1369670	-	954	WP_179990757.1	AraC family transcriptional regulator	-
J4G45_RS07450 (J4G45_07455)	1369837..1371042	+	1206	WP_004973378.1	alkane 1-monooxygenase	-
J4G45_RS07455 (J4G45_07460)	1371219..1371467	+	249	WP_004973383.1	hypothetical protein	Antitoxin
J4G45_RS07460 (J4G45_07465)	1371467..1371802	+	336	WP_096900853.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J4G45_RS07465 (J4G45_07470)	1371892..1373091	-	1200	WP_207973585.1	acyl-CoA dehydrogenase family protein	-
J4G45_RS07470 (J4G45_07475)	1373143..1374453	-	1311	WP_004973388.1	acyl-CoA dehydrogenase family protein	-
J4G45_RS07475 (J4G45_07480)	1374723..1376096	-	1374	WP_004973390.1	AarF/ABC1/UbiB kinase family protein	-
J4G45_RS07480 (J4G45_07485)	1376240..1376545	-	306	WP_154320717.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Antitoxin PemI-like (3-80)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12262.07 Da
• Isoelectric Point: 6.2162

>T196895 WP_096900853.1 NZ_CP071770:1371467-1371802 [Acinetobacter towneri]
MNQGDIYLISLDPTLGHEQQGKRPVFVISPKSFNLITKMPIVLPITSGGNFARNMGFAVELIGGKTTGIVRCDQSRALDF
EARHARFLESSHADVLDEVLARYIAIFSPED

Nucleotide

• Length: 336 bp

>T196895 NZ_CP095510:395516-395701 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8866.38 Da
• Isoelectric Point: 7.3181

>AT196895 WP_004973383.1 NZ_CP071770:1371219-1371467 [Acinetobacter towneri]
MIHTNLRKVGGSVMLAVPPTLLKSLGLDAGKEVSLSIVNGKLVVEPKAKPSYKLADLLQEHQALALEQDAWHDVKAVGRE
EI

Nucleotide

• Length: 249 bp

>AT196895 NZ_CP095510:395718-396128 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: N9CN57