Detailed information of TA system TA196892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 167526..168110
• Replicon: plasmid pGX5
• Accession: NZ_CP071769
• Organism: Acinetobacter towneri strain GX5

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: J4G45_RS00850
• Protein ID: WP_000286964.1
• Coordinates: 167808..168110 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J4G45_RS00845
• Protein ID: WP_000985609.1
• Coordinates: 167526..167807 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G45_RS00820 (J4G45_00815)	163745..164704	+	960	WP_180006246.1	IS30 family transposase	-
J4G45_RS00825 (J4G45_00820)	164815..165087	-	273	WP_000369781.1	NadS family protein	-
J4G45_RS00830 (J4G45_00825)	165080..165400	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J4G45_RS00835 (J4G45_00830)	165540..165959	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
J4G45_RS00840 (J4G45_00835)	166110..167435	+	1326	WP_096902756.1	ISNCY family transposase	-
J4G45_RS00845 (J4G45_00840)	167526..167807	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
J4G45_RS00850 (J4G45_00845)	167808..168110	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G45_RS00855 (J4G45_00850)	168259..168789	+	531	WP_000172759.1	hypothetical protein	-
J4G45_RS00860 (J4G45_00855)	168878..169192	-	315	WP_004728120.1	BrnA antitoxin family protein	-
J4G45_RS13675	169179..169372	-	194	Protein_174	BrnT family toxin	-
J4G45_RS00865 (J4G45_00860)	169351..169728	-	378	Protein_175	AraC family ligand binding domain-containing protein	-
J4G45_RS00870 (J4G45_00865)	169902..170607	-	706	Protein_176	IS1 family transposase	-
J4G45_RS00875 (J4G45_00870)	170697..171539	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
J4G45_RS00880 (J4G45_00875)	171620..171988	+	369	Protein_178	IS1-like element ISAba3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / dfrA20 / tet(X3) / aph(3')-Ia / mph(E) / msr(E) / blaOXA-58	-	1..178808	178808
	-	inside	IScluster/Tn	mph(E) / msr(E) / blaOXA-58	-	145341..173460	28119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T196892 WP_000286964.1 NZ_CP071769:c168110-167808 [Acinetobacter towneri]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T196892 NZ_CP095505:4102396-4102698 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT196892 WP_000985609.1 NZ_CP071769:c167807-167526 [Acinetobacter towneri]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT196892 NZ_CP095505:4102734-4103075 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TV94