Detailed information of TA system TA196889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 7616..8204
• Replicon: plasmid pGX5
• Accession: NZ_CP071769
• Organism: Acinetobacter towneri strain GX5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: J4G45_RS00050
• Protein ID: WP_004647749.1
• Coordinates: 7908..8204 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J4G45_RS00045
• Protein ID: WP_004969304.1
• Coordinates: 7616..7906 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G45_RS00015 (J4G45_00015)	3185..3781	+	597	WP_074163998.1	LysE family transporter	-
J4G45_RS00020 (J4G45_00020)	3809..4627	-	819	WP_179989168.1	AraC family transcriptional regulator	-
J4G45_RS00025 (J4G45_00025)	4758..5039	-	282	WP_000985610.1	putative addiction module antidote protein	-
J4G45_RS00030 (J4G45_00030)	5040..5342	-	303	WP_154320144.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J4G45_RS00035 (J4G45_00035)	5592..5801	+	210	WP_004976362.1	hypothetical protein	-
J4G45_RS00040 (J4G45_00040)	6051..7505	+	1455	WP_026438532.1	SulP family inorganic anion transporter	-
J4G45_RS00045 (J4G45_00045)	7616..7906	-	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
J4G45_RS00050 (J4G45_00050)	7908..8204	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G45_RS13740	8421..8546	+	126	WP_004969301.1	hypothetical protein	-
J4G45_RS00055 (J4G45_00055)	8640..9722	-	1083	WP_004969299.1	hypothetical protein	-
J4G45_RS00060 (J4G45_00060)	9891..11216	+	1326	WP_179989167.1	ISNCY family transposase	-
J4G45_RS00065 (J4G45_00065)	11683..12249	+	567	WP_006581699.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / sul2 / dfrA20 / tet(X3) / aph(3')-Ia / mph(E) / msr(E) / blaOXA-58	-	1..178808	178808

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T196889 WP_004647749.1 NZ_CP071769:c8204-7908 [Acinetobacter towneri]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T196889 NZ_CP095505:3639147-3639461 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT196889 WP_004969304.1 NZ_CP071769:c7906-7616 [Acinetobacter towneri]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT196889 NZ_CP095505:3638911-3639144 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG