Detailed information of TA system TA196883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 93665..94253
• Replicon: plasmid pGX3-1
• Accession: NZ_CP071767
• Organism: Acinetobacter towneri strain GX3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: J4G44_RS12955
• Protein ID: WP_004647749.1
• Coordinates: 93665..93961 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J4G44_RS12960
• Protein ID: WP_004969304.1
• Coordinates: 93963..94253 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G44_RS12940 (J4G44_12940)	89620..90186	-	567	WP_006581699.1	recombinase family protein	-
J4G44_RS12945 (J4G44_12945)	90653..91978	-	1326	WP_179989167.1	ISNCY family transposase	-
J4G44_RS12950 (J4G44_12950)	92147..93229	+	1083	WP_004969299.1	hypothetical protein	-
J4G44_RS13620	93323..93448	-	126	WP_004969301.1	hypothetical protein	-
J4G44_RS12955 (J4G44_12955)	93665..93961	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G44_RS12960 (J4G44_12960)	93963..94253	+	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
J4G44_RS12965 (J4G44_12965)	94364..95818	-	1455	WP_026438532.1	SulP family inorganic anion transporter	-
J4G44_RS12970 (J4G44_12970)	96068..96277	-	210	WP_004976362.1	hypothetical protein	-
J4G44_RS12975 (J4G44_12975)	96527..96829	+	303	WP_154320144.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J4G44_RS12980 (J4G44_12980)	96830..97111	+	282	WP_000985610.1	putative addiction module antidote protein	-
J4G44_RS12985 (J4G44_12985)	97242..98060	+	819	WP_179989168.1	AraC family transcriptional regulator	-
J4G44_RS12990 (J4G44_12990)	98088..98684	-	597	WP_074163998.1	LysE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / tet(X3) / sul2 / dfrA20 / floR / blaOXA-58 / msr(E) / mph(E)	-	1..148166	148166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T196883 WP_004647749.1 NZ_CP071767:93665-93961 [Acinetobacter towneri]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T196883 NZ_CP095505:2845234-2845545 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT196883 WP_004969304.1 NZ_CP071767:93963-94253 [Acinetobacter towneri]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT196883 NZ_CP095505:2845546-2845836 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG