Detailed information of TA system TA196878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1115165..1115814
• Replicon: chromosome
• Accession: NZ_CP071766
• Organism: Acinetobacter towneri strain GX3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: N9B9L0
• Locus tag: J4G44_RS05295
• Protein ID: WP_004972405.1
• Coordinates: 1115395..1115814 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: N9CPX4
• Locus tag: J4G44_RS05290
• Protein ID: WP_004972406.1
• Coordinates: 1115165..1115395 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G44_RS05275 (J4G44_05275)	1110285..1111091	-	807	WP_026438287.1	tetratricopeptide repeat protein	-
J4G44_RS05280 (J4G44_05280)	1111283..1112005	-	723	WP_004972410.1	folate-binding protein YgfZ	-
J4G44_RS05285 (J4G44_05285)	1112262..1114868	+	2607	WP_096901558.1	Fe/S-dependent 2-methylisocitrate dehydratase AcnD	-
J4G44_RS05290 (J4G44_05290)	1115165..1115395	+	231	WP_004972406.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J4G44_RS05295 (J4G44_05295)	1115395..1115814	+	420	WP_004972405.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4G44_RS05300 (J4G44_05300)	1115945..1116088	+	144	WP_096901556.1	hypothetical protein	-
J4G44_RS05305 (J4G44_05305)	1116196..1116768	+	573	WP_019837767.1	DUF4126 domain-containing protein	-
J4G44_RS05310 (J4G44_05310)	1117332..1117583	+	252	WP_004972400.1	hypothetical protein	-
J4G44_RS05315 (J4G44_05315)	1117703..1118416	+	714	WP_004972399.1	SIMPL domain-containing protein	-
J4G44_RS05320 (J4G44_05320)	1118568..1119233	+	666	WP_180059923.1	pseudouridine synthase	-
J4G44_RS05325 (J4G44_05325)	1119513..1120463	+	951	WP_228291706.1	DUF5660 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-128)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 16147.60 Da
• Isoelectric Point: 7.1858

>T196878 WP_004972405.1 NZ_CP071766:1115395-1115814 [Acinetobacter towneri]
MQVQYLLDTNICIYIIKYRPEIVRQHFEKHLPHRNILISVITLGELRFGAEKSQHKEKALKVIDELTSMIQVVELDENVA
HHYAEIRQNLASKGQIIGANDLWLAAHARANHWVMVTNNEKEFTRVDGLKVENWVSNPL

Nucleotide

• Length: 420 bp

>T196878 NZ_CP095505:c648445-648194 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9068.42 Da
• Isoelectric Point: 5.1972

>AT196878 WP_004972406.1 NZ_CP071766:1115165-1115395 [Acinetobacter towneri]
MTTAKVFMTGRSQAIRLPKEFRFEGKEVEIFRRGDEVILREKPVTVDRLFKALSQMPNDFYADEREDLPPQEREMF

Nucleotide

• Length: 231 bp

>AT196878 NZ_CP095505:c648197-647943 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCACTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: N9B9L0

Antitoxin

• AlphaFold DB: N9CPX4