Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 3222807..3224563 Replicon chromosome
Accession NZ_CP071762
Organism Burkholderia pseudomallei strain VBP23831

Toxin (Protein)


Gene name doc Uniprot ID C4APV8
Locus tag J3L14_RS31330 Protein ID WP_004195452.1
Coordinates 3222807..3223712 (+) Length 302 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q63LW2
Locus tag J3L14_RS31335 Protein ID WP_004195450.1
Coordinates 3223709..3224563 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3L14_RS31300 (J3L14_31300) 3218202..3219290 - 1089 WP_004184835.1 porin -
J3L14_RS31305 (J3L14_31305) 3219725..3220042 + 318 WP_004184916.1 non-heme iron oxygenase ferredoxin subunit -
J3L14_RS31310 (J3L14_31310) 3220044..3220355 + 312 WP_004184841.1 hypothetical protein -
J3L14_RS31315 (J3L14_31315) 3220390..3221457 + 1068 WP_004195457.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
J3L14_RS31320 (J3L14_31320) 3221454..3222224 + 771 WP_004524000.1 SDR family oxidoreductase -
J3L14_RS31325 (J3L14_31325) 3222263..3222790 + 528 WP_004524001.1 cupin domain-containing protein -
J3L14_RS31330 (J3L14_31330) 3222807..3223712 + 906 WP_004195452.1 alpha/beta fold hydrolase Toxin
J3L14_RS31335 (J3L14_31335) 3223709..3224563 + 855 WP_004195450.1 IclR family transcriptional regulator Antitoxin
J3L14_RS31340 (J3L14_31340) 3224560..3225357 + 798 WP_004540865.1 SDR family oxidoreductase -
J3L14_RS31345 (J3L14_31345) 3225350..3227023 + 1674 WP_207877638.1 thiamine pyrophosphate-binding protein -
J3L14_RS31350 (J3L14_31350) 3227016..3227831 + 816 WP_004195445.1 aspartate dehydrogenase -
J3L14_RS31355 (J3L14_31355) 3227857..3229347 + 1491 WP_004549581.1 aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(54-284)

Antitoxin

Transcription regulator IclR, C-terminal

(143-265)


Sequences

Toxin        


Download         Length: 302 a.a.        Molecular weight: 30555.70 Da        Isoelectric Point: 8.7357

>T196872 WP_004195452.1 NZ_CP071762:3222807-3223712 [Burkholderia pseudomallei]
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Download         Length: 906 bp

>T196872 NZ_CP095502:14061-14210 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31152.56 Da        Isoelectric Point: 6.8932

>AT196872 WP_004195450.1 NZ_CP071762:3223709-3224563 [Burkholderia pseudomallei]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFDVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR

Download         Length: 855 bp

>AT196872 NZ_CP095502:c14013-13957 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N4FRK3

Antitoxin

Source ID Structure
AlphaFold DB A0A3N4FRW8

References