Detailed information of TA system TA196864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 748400..750153
• Replicon: chromosome
• Accession: NZ_CP071760
• Organism: Burkholderia pseudomallei strain VBP21885

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: J3J51_RS21105
• Protein ID: WP_004188815.1
• Coordinates: 748801..750153 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J3J51_RS21100
• Protein ID: WP_004188446.1
• Coordinates: 748400..748804 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3J51_RS21090 (J3J51_21090)	744938..747334	-	2397	WP_045588572.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
J3J51_RS21095 (J3J51_21095)	747799..748062	+	264	WP_004199789.1	hypothetical protein	-
J3J51_RS21100 (J3J51_21100)	748400..748804	+	405	WP_004188446.1	helix-turn-helix transcriptional regulator	Antitoxin
J3J51_RS21105 (J3J51_21105)	748801..750153	+	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3J51_RS21110 (J3J51_21110)	750332..750784	-	453	WP_004198842.1	hypothetical protein	-
J3J51_RS21115 (J3J51_21115)	750885..751031	-	147	WP_004199790.1	hypothetical protein	-
J3J51_RS21120 (J3J51_21120)	751187..752101	-	915	WP_004545706.1	LysR family transcriptional regulator	-
J3J51_RS21125 (J3J51_21125)	752268..753221	+	954	WP_004187785.1	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T196864 WP_004188815.1 NZ_CP071760:748801-750153 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T196864 NZ_CP095497:c964521-964204 [Burkholderia cepacia]
ATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTTTGCCGGCCTGCGCGATCGCGT
CGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCGGGCTCGCCGG
TCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCATCCTGCTC
GGCGGCGGCGATACATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGCGCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14392.75 Da
• Isoelectric Point: 11.7233

>AT196864 WP_004188446.1 NZ_CP071760:748400-748804 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTVANARAGARRAVPPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT196864 NZ_CP095497:c964200-963901 [Burkholderia cepacia]
ATGAACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGATTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCCTGCATGA

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2