Detailed information of TA system TA196863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 32005..32660
• Replicon: chromosome
• Accession: NZ_CP071760
• Organism: Burkholderia pseudomallei strain VBP21885

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J3J51_RS18235
• Protein ID: WP_024431052.1
• Coordinates: 32005..32301 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: J3J51_RS18240
• Protein ID: WP_004202809.1
• Coordinates: 32304..32660 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3J51_RS18200 (J3J51_18200)	27345..27611	+	267	WP_004532697.1	ogr/Delta-like zinc finger family protein	-
J3J51_RS18205 (J3J51_18205)	27608..27814	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
J3J51_RS18210 (J3J51_18210)	27904..28557	+	654	WP_004532770.1	AAA family ATPase	-
J3J51_RS31485	28632..28913	+	282	WP_015985021.1	hypothetical protein	-
J3J51_RS18220 (J3J51_18220)	29016..29549	+	534	WP_004532793.1	PAAR domain-containing protein	-
J3J51_RS18225 (J3J51_18225)	29546..30283	+	738	WP_004532838.1	VRR-NUC domain-containing protein	-
J3J51_RS18230 (J3J51_18230)	30298..31413	+	1116	WP_029670743.1	DUF3396 domain-containing protein	-
J3J51_RS18235 (J3J51_18235)	32005..32301	+	297	WP_024431052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3J51_RS18240 (J3J51_18240)	32304..32660	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
J3J51_RS18245 (J3J51_18245)	32704..33759	-	1056	WP_207866379.1	phage portal protein	-
J3J51_RS18250 (J3J51_18250)	33756..35525	-	1770	WP_207874575.1	terminase ATPase subunit family protein	-
J3J51_RS18255 (J3J51_18255)	35669..36478	+	810	WP_004532870.1	GPO family capsid scaffolding protein	-
J3J51_RS18260 (J3J51_18260)	36512..37525	+	1014	WP_004531048.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	18761..56320	37559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10916.71 Da
• Isoelectric Point: 9.9369

>T196863 WP_024431052.1 NZ_CP071760:32005-32301 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRREKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T196863 NZ_CP095497:c964200-963901 [Burkholderia cepacia]
ATGAACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGATTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCCTGCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT196863 WP_004202809.1 NZ_CP071760:32304-32660 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT196863 NZ_CP095497:c964521-964204 [Burkholderia cepacia]
ATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTTTGCCGGCCTGCGCGATCGCGT
CGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCGGGCTCGCCGG
TCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCATCCTGCTC
GGCGGCGGCGATACATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGCGCACCTCGACATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3