Detailed information of TA system TA196861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 124315..124948
• Replicon: chromosome
• Accession: NZ_CP071759
• Organism: Burkholderia pseudomallei strain VBP21885

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3J51_RS00485
• Protein ID: WP_207866055.1
• Coordinates: 124315..124728 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A105KNA0
• Locus tag: J3J51_RS00490
• Protein ID: WP_042583866.1
• Coordinates: 124715..124948 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3J51_RS00460 (J3J51_00460)	119675..120616	+	942	WP_207866065.1	TIGR03756 family integrating conjugative element protein	-
J3J51_RS00465 (J3J51_00465)	120625..122040	+	1416	WP_207866063.1	integrating conjugative element protein	-
J3J51_RS00470 (J3J51_00470)	122043..122384	+	342	WP_207866061.1	hypothetical protein	-
J3J51_RS00475 (J3J51_00475)	122398..123903	+	1506	WP_207866059.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
J3J51_RS00480 (J3J51_00480)	123913..124281	-	369	WP_207866057.1	DUF3742 family protein	-
J3J51_RS00485 (J3J51_00485)	124315..124728	-	414	WP_207866055.1	PIN domain-containing protein	Toxin
J3J51_RS00490 (J3J51_00490)	124715..124948	-	234	WP_042583866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3J51_RS00495 (J3J51_00495)	125437..125904	+	468	WP_207866053.1	hypothetical protein	-
J3J51_RS00500 (J3J51_00500)	125938..127596	-	1659	WP_207866051.1	MASE1 domain-containing protein	-
J3J51_RS00505 (J3J51_00505)	127909..129741	+	1833	WP_207866049.1	MobH family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	37446..141875	104429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15367.81 Da
• Isoelectric Point: 6.0839

>T196861 WP_207866055.1 NZ_CP071759:c124728-124315 [Burkholderia pseudomallei]
MSGADKTKVFVDSNVVLYLLSGDTAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVEQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Nucleotide

• Length: 414 bp

>T196861 NZ_CP095476:c4344492-4343746 [Bacillus glycinifermentans]
ATGCTGCTTTTTTATCAGTTTTCAGTCTGGGTGATCGTCCTCGCCTTAGCCTTGTACGTTTTTGCCGTCTGGCGTTTTGA
AAAAAAGATGAAAGAAAAAACGATCGCGATCCGGAAAACGTGGTATCTCCTTTATGTGATCGGCGCCGCGCTTTACTGGA
CGTACGATCCCCAATCCATTTTCAGCAATTGGCTCCACTACTTGATCGTCGCCGTCTTTTTCACGCTGACCGATGCGTTT
ATTTTCTTAAACGCCTACTTCAAAAAGCTCGGCACGAACGAATTGGCCACAGACACAAGAGAACTTCTTGAAGAAAACAA
CGACCTGCTTCACACGTATCAAAACAGGCTCAAAACGTACCAATACCTATTGAAAAACGAACCGATCCACATCTACTATG
GAGATATAGAAGCTTACGCTGAAGGCATTGAAAAACTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCCGCACTA
TGCGAATATAATTCGAACGAGAGCAAAGATCATTTGCTCGAACATATGGAAAACCGTTTCGCTGTTCAGGAAAAGCTGGA
CCGGAAAGACGTGTATTACGAAGAAAACGGAAAAATGGTCCTGATTCCATTTTCGATCCATGAATACGACTATGTGATGA
AACTGACATCCGAGGACCTTGTCACTGAATTTGATTATCTGCTGTTTACTTCTTTAACCAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8752.02 Da
• Isoelectric Point: 8.7841

>AT196861 WP_042583866.1 NZ_CP071759:c124948-124715 [Burkholderia pseudomallei]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRRFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT196861 NZ_CP095476:c4343746-4343573 [Bacillus glycinifermentans]
ATGGAACCGGTTTTTCAGCAAGACAAAGACCGTTCAACGGGGCGCGGCACATGTAAAGTATTCAAAAAACAATCAAAAAC
CCACATCGCCAAATATGAGGTCAGCCCACACACCGAACGGATTTTCAGGCAAAATGCCCGGCTGATCGATGAGTATAAAC
GAAAAAATGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A105KNA0