Detailed information of TA system TA196860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2451603..2453344
• Replicon: chromosome
• Accession: NZ_CP071758
• Organism: Burkholderia pseudomallei strain VBP399

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: J3E55_RS28215
• Protein ID: WP_004188815.1
• Coordinates: 2451603..2452955 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J3E55_RS28220
• Protein ID: WP_017881212.1
• Coordinates: 2452952..2453344 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3E55_RS28190 (J3E55_28190)	2447902..2448240	-	339	WP_004188037.1	hypothetical protein	-
J3E55_RS28195 (J3E55_28195)	2448516..2449469	-	954	WP_004187785.1	agmatinase	-
J3E55_RS28200 (J3E55_28200)	2449636..2450550	+	915	WP_004198844.1	LysR family transcriptional regulator	-
J3E55_RS28205 (J3E55_28205)	2450725..2450871	+	147	WP_004199790.1	hypothetical protein	-
J3E55_RS28210 (J3E55_28210)	2450972..2451424	+	453	WP_004198842.1	hypothetical protein	-
J3E55_RS28215 (J3E55_28215)	2451603..2452955	-	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3E55_RS28220 (J3E55_28220)	2452952..2453344	-	393	WP_017881212.1	helix-turn-helix domain-containing protein	Antitoxin
J3E55_RS28225 (J3E55_28225)	2453682..2453945	-	264	WP_004199789.1	hypothetical protein	-
J3E55_RS28230 (J3E55_28230)	2454411..2456807	+	2397	WP_122657833.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T196860 WP_004188815.1 NZ_CP071758:c2452955-2451603 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T196860 NZ_CP095476:3569528-3569878 [Bacillus glycinifermentans]
TTGATTGTTAAACGCGGCGATGTCTATTTTGCTGATCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTATGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATC
CGGACGATTGATAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTTAACGAAGCCTT
ACAAATCAGTTTGGCACTAATCGATTTTTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13884.08 Da
• Isoelectric Point: 11.5433

>AT196860 WP_017881212.1 NZ_CP071758:c2453344-2452952 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGNDKGEVAKTAAGARRAAPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 393 bp

>AT196860 NZ_CP095476:3569242-3569523 [Bacillus glycinifermentans]
TTGTCTGAATCCAGCGCTACAACTGAAATTATGATTCGCTTGCCAGAAGCCTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCCGAAATGAACTGATTTATCAAGCCACAAAAATGTATTTACGTGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCAAAAATCAATTTAAACATCTCCTCAGAGGCACAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2