Detailed information of TA system TA196859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 886429..886900
• Replicon: chromosome
• Accession: NZ_CP071758
• Organism: Burkholderia pseudomallei strain VBP399

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: J3E55_RS21705
• Protein ID: WP_229288201.1
• Coordinates: 886697..886900 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: J3E55_RS21700
• Protein ID: WP_004525420.1
• Coordinates: 886429..886713 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3E55_RS21675 (J3E55_21675)	881518..882888	-	1371	WP_076811855.1	cytochrome c peroxidase	-
J3E55_RS21680 (J3E55_21680)	882939..884399	-	1461	WP_004538070.1	metallophosphoesterase	-
J3E55_RS21685 (J3E55_21685)	884665..884781	+	117	Protein_717	transposase	-
J3E55_RS21690 (J3E55_21690)	885008..885400	-	393	Protein_718	IS110 family transposase	-
J3E55_RS21695 (J3E55_21695)	885399..885782	-	384	Protein_719	integrase core domain-containing protein	-
J3E55_RS21700 (J3E55_21700)	886429..886713	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
J3E55_RS21705 (J3E55_21705)	886697..886900	-	204	WP_229288201.1	BrnT family toxin	Toxin
J3E55_RS21710 (J3E55_21710)	887538..887813	+	276	WP_009975384.1	hypothetical protein	-
J3E55_RS21715 (J3E55_21715)	887823..887954	+	132	WP_009923452.1	bacteriophage protein Gp48	-
J3E55_RS21720 (J3E55_21720)	888090..888311	+	222	WP_004552390.1	hypothetical protein	-
J3E55_RS21725 (J3E55_21725)	888278..888577	+	300	WP_160458000.1	hypothetical protein	-
J3E55_RS21730 (J3E55_21730)	888808..889389	-	582	WP_004542548.1	hypothetical protein	-
J3E55_RS21735 (J3E55_21735)	889648..889797	+	150	WP_004537646.1	bacteriophage protein Gp44	-
J3E55_RS21740 (J3E55_21740)	889833..890366	+	534	Protein_728	SPFH domain-containing protein	-
J3E55_RS21745 (J3E55_21745)	890565..890939	+	375	Protein_729	integrase core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-59)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7762.71 Da
• Isoelectric Point: 7.9951

>T196859 WP_229288201.1 NZ_CP071758:c886900-886697 [Burkholderia pseudomallei]
MQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 204 bp

>T196859 NZ_CP095475:c3822768-3822487 [Pseudoxanthomonas winnipegensis]
GTGGCCATCCAGTCCTTCGCCGACAAGCACACCCAGCACCTTTTCGAAGGCCGGCGCGTGCGGCGCTGGATCAACGTTGA
AGTGGTCGCGATGCGCAAGCTGGCAATGCTCAACGCGGCAGCGGTGCTGGATGACCTGCGTATTCCTCCCGCCAACCGGC
TGGAGGCTTTACAGGGCGATCGTCGCGGCCAGCACAGCATCCGCATCAACGACCAGTACCGGGTGTGCTTCGTCTGGACC
CCGACCGGTCCGGCGAACGTCGAAATCGTCGACTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT196859 WP_004525420.1 NZ_CP071758:c886713-886429 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT196859 NZ_CP095475:c3822475-3822179 [Pseudoxanthomonas winnipegensis]
ATGCGCACCATCGCTTATCCCAAGCCCGGCGAGATCCTGCTACACGAGTTTCTCGAGCCCATGGGCATCACCCAGTACCG
TTTGGCCAAGGCCATCGGCGTGCCGCAGCGGCGCATCGGCGAGATCGTCGGCGGCAATCGCGGCGTGACCGCGGATACCG
CCCTGCGCCTGTCGCGCTACTTCGGCACCTCTGTCGGGTTCTGGTTGGGCCTGCAGATGGACCACGACGCGGCCGTGGCG
CGCGACGCGCTGGCCGAGACGCTGGCAAAAATCGAACCGTACCGGCCAGCAGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8