196857

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 3053138..3053675 Replicon chromosome
Accession NZ_CP071755
Organism Burkholderia pseudomallei strain VBP364

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag J3D99_RS30445 Protein ID WP_004549731.1
Coordinates 3053406..3053675 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag J3D99_RS30440 Protein ID WP_004525420.1
Coordinates 3053138..3053422 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3D99_RS30415 (J3D99_019250) 3048246..3049508 - 1263 WP_004551329.1 cytochrome c peroxidase -
J3D99_RS30420 (J3D99_019255) 3049667..3051127 - 1461 WP_011326543.1 metallophosphoesterase -
J3D99_RS30425 (J3D99_019260) 3051374..3051490 + 117 Protein_2484 IS3 family transposase -
J3D99_RS30430 (J3D99_019265) 3051717..3052109 - 393 Protein_2485 IS110 family transposase -
J3D99_RS30435 (J3D99_019270) 3052108..3052491 - 384 Protein_2486 integrase core domain-containing protein -
J3D99_RS30440 (J3D99_019275) 3053138..3053422 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
J3D99_RS30445 (J3D99_019280) 3053406..3053675 - 270 WP_004549731.1 BrnT family toxin Toxin
J3D99_RS30450 (J3D99_019285) 3054246..3054521 + 276 WP_207416682.1 hypothetical protein -
J3D99_RS30455 (J3D99_019290) 3054531..3054662 + 132 WP_009923452.1 bacteriophage protein Gp48 -
J3D99_RS30460 (J3D99_019295) 3054800..3055042 + 243 WP_207416680.1 hypothetical protein -
J3D99_RS30465 (J3D99_019300) 3055039..3055197 + 159 WP_004549802.1 hypothetical protein -
J3D99_RS30470 (J3D99_019305) 3055245..3055463 + 219 WP_004552392.1 hypothetical protein -
J3D99_RS30475 (J3D99_019310) 3055649..3056302 - 654 WP_004545536.1 hypothetical protein -
J3D99_RS30480 (J3D99_019315) 3056551..3056700 + 150 WP_004537646.1 bacteriophage protein Gp44 -
J3D99_RS30485 (J3D99_019320) 3056736..3057269 + 534 Protein_2496 SPFH domain-containing protein -
J3D99_RS30490 (J3D99_019325) 3057468..3057896 + 429 Protein_2497 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3046024..3074543 28519


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T196857 WP_004549731.1 NZ_CP071755:c3053675-3053406 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T196857 NZ_CP095475:c1977482-1977192 [Pseudoxanthomonas winnipegensis]
ATGCCACACCTGAGCTGGGCGCCCGCCGCAGTCTCAGATATTCAACGCCTTTACCGCTTCCTGCTGCCCAAGAATGCCGA
CGCCGCCCGGCGCGCCATCAAGGCCATCCGCGAGGGCGTCAAGATCCTCGCCCGGCAGCCACGCATCGGACGGCCCATCG
AAGACCTGCCAACGGAATTCCGCGACTGGCCGATCGATTTCGGCGACGATGGGTATGTGGTCCGATATCGGATCGAGAAT
GAGGTAGTGACAATCCTGGCGGTGCGACACCAGAAGGAAGCTGGATTTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT196857 WP_004525420.1 NZ_CP071755:c3053422-3053138 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT196857 NZ_CP095475:c1977748-1977470 [Pseudoxanthomonas winnipegensis]
ATGGCATCGCCGACCTCGCTCAAGCTCGATCAAGCGATGAAAGAGCGCATTCAGCACCTGGCGGACACCCGTCGCCGGTC
CCCGCACTGGATCATGCGCGAGGCCATCGCACAGTACGTCGAACGCGAGGAGCAGCGCGAGGCCTTCCGTCAGGACACGC
TGCGCGCATGGGAGGAGTTCCAGCGGACCGGCCTGCATCTGACCGGAGCCGAAGCCGACGCGTGGCTGGCCAGGCTCGAA
GCGGGTGAGGACGCGGAGCCGCCCGCATGCCACACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References