Detailed information of TA system TA196849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 186932..188685
• Replicon: chromosome
• Accession: NZ_CP071752
• Organism: Burkholderia pseudomallei strain VBM23831

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J3C54_RS18060
• Protein ID: WP_207876368.1
• Coordinates: 186932..188284 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J3C54_RS18065
• Protein ID: WP_004188446.1
• Coordinates: 188281..188685 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3C54_RS18040 (J3C54_18040)	183875..184828	-	954	WP_004187785.1	agmatinase	-
J3C54_RS18045 (J3C54_18045)	184995..185909	+	915	WP_004545706.1	LysR family transcriptional regulator	-
J3C54_RS18050 (J3C54_18050)	186065..186211	+	147	WP_004199790.1	hypothetical protein	-
J3C54_RS18055 (J3C54_18055)	186312..186764	+	453	WP_004198842.1	hypothetical protein	-
J3C54_RS18060 (J3C54_18060)	186932..188284	-	1353	WP_207876368.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3C54_RS18065 (J3C54_18065)	188281..188685	-	405	WP_004188446.1	helix-turn-helix transcriptional regulator	Antitoxin
J3C54_RS18070 (J3C54_18070)	189023..189286	-	264	WP_004199789.1	hypothetical protein	-
J3C54_RS18075 (J3C54_18075)	189752..192148	+	2397	WP_207876369.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (11-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49838.80 Da
• Isoelectric Point: 9.6807

>T196849 WP_207876368.1 NZ_CP071752:c188284-186932 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDSQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T196849 NZ_CP095452:7053-7307 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14392.75 Da
• Isoelectric Point: 11.7233

>AT196849 WP_004188446.1 NZ_CP071752:c188685-188281 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTVANARAGARRAVPPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT196849 NZ_CP095452:7304-7546 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA