Detailed information of TA system TA196846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3124053..3125806
• Replicon: chromosome
• Accession: NZ_CP071750
• Organism: Burkholderia pseudomallei strain VBM21885

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: J3B47_RS31145
• Protein ID: WP_004188815.1
• Coordinates: 3124454..3125806 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J3B47_RS31140
• Protein ID: WP_004188446.1
• Coordinates: 3124053..3124457 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3B47_RS31130 (J3B47_31130)	3120591..3122987	-	2397	WP_045588572.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
J3B47_RS31135 (J3B47_31135)	3123452..3123715	+	264	WP_004199789.1	hypothetical protein	-
J3B47_RS31140 (J3B47_31140)	3124053..3124457	+	405	WP_004188446.1	helix-turn-helix transcriptional regulator	Antitoxin
J3B47_RS31145 (J3B47_31145)	3124454..3125806	+	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3B47_RS31150 (J3B47_31150)	3125985..3126437	-	453	WP_004198842.1	hypothetical protein	-
J3B47_RS31155 (J3B47_31155)	3126538..3126684	-	147	WP_004199790.1	hypothetical protein	-
J3B47_RS31160 (J3B47_31160)	3126840..3127754	-	915	WP_004545706.1	LysR family transcriptional regulator	-
J3B47_RS31165 (J3B47_31165)	3127921..3128874	+	954	WP_004187785.1	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T196846 WP_004188815.1 NZ_CP071750:3124454-3125806 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T196846 NZ_CP095448:4604523-4604900 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14392.75 Da
• Isoelectric Point: 11.7233

>AT196846 WP_004188446.1 NZ_CP071750:3124053-3124457 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTVANARAGARRAVPPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT196846 NZ_CP095448:4604102-4604476 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2