Detailed information of TA system TA196845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2407651..2408306
• Replicon: chromosome
• Accession: NZ_CP071750
• Organism: Burkholderia pseudomallei strain VBM21885

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J3B47_RS28270
• Protein ID: WP_024431052.1
• Coordinates: 2407651..2407947 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: J3B47_RS28275
• Protein ID: WP_004202809.1
• Coordinates: 2407950..2408306 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3B47_RS28235 (J3B47_28235)	2402991..2403257	+	267	WP_004532697.1	ogr/Delta-like zinc finger family protein	-
J3B47_RS28240 (J3B47_28240)	2403254..2403460	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
J3B47_RS28245 (J3B47_28245)	2403550..2404203	+	654	WP_004532770.1	AAA family ATPase	-
J3B47_RS32295	2404278..2404559	+	282	WP_015985021.1	hypothetical protein	-
J3B47_RS28255 (J3B47_28255)	2404662..2405195	+	534	WP_004532793.1	PAAR domain-containing protein	-
J3B47_RS28260 (J3B47_28260)	2405192..2405929	+	738	WP_004532838.1	VRR-NUC domain-containing protein	-
J3B47_RS28265 (J3B47_28265)	2405944..2407059	+	1116	WP_029670743.1	DUF3396 domain-containing protein	-
J3B47_RS28270 (J3B47_28270)	2407651..2407947	+	297	WP_024431052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3B47_RS28275 (J3B47_28275)	2407950..2408306	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
J3B47_RS28280 (J3B47_28280)	2408350..2409405	-	1056	WP_207866379.1	phage portal protein	-
J3B47_RS28285 (J3B47_28285)	2409402..2411171	-	1770	WP_207865891.1	terminase ATPase subunit family protein	-
J3B47_RS28290 (J3B47_28290)	2411315..2412124	+	810	WP_004532870.1	GPO family capsid scaffolding protein	-
J3B47_RS28295 (J3B47_28295)	2412158..2413171	+	1014	WP_004531048.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2394407..2431966	37559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10916.71 Da
• Isoelectric Point: 9.9369

>T196845 WP_024431052.1 NZ_CP071750:2407651-2407947 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRREKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T196845 NZ_CP095448:c4447254-4446904 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT196845 WP_004202809.1 NZ_CP071750:2407950-2408306 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT196845 NZ_CP095448:c4447499-4447248 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3