Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/HTH_IclR(antitoxin) |
| Location | 2159873..2161629 | Replicon | chromosome |
| Accession | NZ_CP071750 | ||
| Organism | Burkholderia pseudomallei strain VBM21885 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | C4APV8 |
| Locus tag | J3B47_RS27395 | Protein ID | WP_004195452.1 |
| Coordinates | 2159873..2160778 (+) | Length | 302 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | Q63LW2 |
| Locus tag | J3B47_RS27400 | Protein ID | WP_004195450.1 |
| Coordinates | 2160775..2161629 (+) | Length | 285 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J3B47_RS27365 (J3B47_27365) | 2155268..2156356 | - | 1089 | WP_004184835.1 | porin | - |
| J3B47_RS27370 (J3B47_27370) | 2156791..2157108 | + | 318 | WP_004184916.1 | non-heme iron oxygenase ferredoxin subunit | - |
| J3B47_RS27375 (J3B47_27375) | 2157110..2157421 | + | 312 | WP_004184841.1 | hypothetical protein | - |
| J3B47_RS27380 (J3B47_27380) | 2157456..2158523 | + | 1068 | WP_004195457.1 | aromatic ring-hydroxylating dioxygenase subunit alpha | - |
| J3B47_RS27385 (J3B47_27385) | 2158520..2159290 | + | 771 | WP_004524000.1 | SDR family oxidoreductase | - |
| J3B47_RS27390 (J3B47_27390) | 2159329..2159856 | + | 528 | WP_004524001.1 | cupin domain-containing protein | - |
| J3B47_RS27395 (J3B47_27395) | 2159873..2160778 | + | 906 | WP_004195452.1 | alpha/beta fold hydrolase | Toxin |
| J3B47_RS27400 (J3B47_27400) | 2160775..2161629 | + | 855 | WP_004195450.1 | IclR family transcriptional regulator | Antitoxin |
| J3B47_RS27405 (J3B47_27405) | 2161626..2162423 | + | 798 | WP_004540865.1 | SDR family oxidoreductase | - |
| J3B47_RS27410 (J3B47_27410) | 2162416..2164089 | + | 1674 | WP_004524004.1 | thiamine pyrophosphate-binding protein | - |
| J3B47_RS27415 (J3B47_27415) | 2164082..2164897 | + | 816 | WP_004195445.1 | aspartate dehydrogenase | - |
| J3B47_RS27420 (J3B47_27420) | 2164923..2166413 | + | 1491 | WP_004549581.1 | aldehyde dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 302 a.a. Molecular weight: 30555.70 Da Isoelectric Point: 8.7357
>T196844 WP_004195452.1 NZ_CP071750:2159873-2160778 [Burkholderia pseudomallei]
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP
Download Length: 906 bp
>T196844 NZ_CP095448:c4435850-4435560 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA
Antitoxin
Download Length: 285 a.a. Molecular weight: 31152.56 Da Isoelectric Point: 6.8932
>AT196844 WP_004195450.1 NZ_CP071750:2160775-2161629 [Burkholderia pseudomallei]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFDVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFDVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR
Download Length: 855 bp
>AT196844 NZ_CP095448:c4436082-4435840 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3N4FRK3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3N4FRW8 |