Detailed information of TA system TA196842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1498333..1498966
• Replicon: chromosome
• Accession: NZ_CP071749
• Organism: Burkholderia pseudomallei strain VBM21885

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3B47_RS06965
• Protein ID: WP_207866055.1
• Coordinates: 1498553..1498966 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A105KNA0
• Locus tag: J3B47_RS06960
• Protein ID: WP_042583866.1
• Coordinates: 1498333..1498566 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3B47_RS06945 (J3B47_06945)	1493540..1495372	-	1833	WP_207866049.1	MobH family relaxase	-
J3B47_RS06950 (J3B47_06950)	1495685..1497343	+	1659	WP_207866051.1	MASE1 domain-containing protein	-
J3B47_RS06955 (J3B47_06955)	1497377..1497844	-	468	WP_207866053.1	hypothetical protein	-
J3B47_RS06960 (J3B47_06960)	1498333..1498566	+	234	WP_042583866.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3B47_RS06965 (J3B47_06965)	1498553..1498966	+	414	WP_207866055.1	PIN domain-containing protein	Toxin
J3B47_RS06970 (J3B47_06970)	1499000..1499368	+	369	WP_207866057.1	DUF3742 family protein	-
J3B47_RS06975 (J3B47_06975)	1499378..1500883	-	1506	WP_207866059.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
J3B47_RS06980 (J3B47_06980)	1500897..1501238	-	342	WP_207866061.1	hypothetical protein	-
J3B47_RS06985 (J3B47_06985)	1501241..1502656	-	1416	WP_207866063.1	integrating conjugative element protein	-
J3B47_RS06990 (J3B47_06990)	1502665..1503606	-	942	WP_207866065.1	TIGR03756 family integrating conjugative element protein	-
J3B47_RS06995 (J3B47_06995)	1503603..1503926	-	324	WP_243435420.1	TIGR03757 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1481406..1585835	104429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15367.81 Da
• Isoelectric Point: 6.0839

>T196842 WP_207866055.1 NZ_CP071749:1498553-1498966 [Burkholderia pseudomallei]
MSGADKTKVFVDSNVVLYLLSGDTAKADRAEALLRRRPTISVQVLNEVTNVCVRKLGMAWREVEQFLELVRQFCVVAPLT
IEMHDLARRIAERYGLAFYDACIVAAASIEGCHALYTEDMHHGLIIEESLTLRNPFA

Nucleotide

• Length: 414 bp

>T196842 NZ_CP095448:4278105-4278446 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8752.02 Da
• Isoelectric Point: 8.7841

>AT196842 WP_042583866.1 NZ_CP071749:1498333-1498566 [Burkholderia pseudomallei]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVIDAPRTFAVRKKPGADELLERLRRFRGKLPADFKFSRDEANERG

Nucleotide

• Length: 234 bp

>AT196842 NZ_CP095448:c4278110-4278034 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A105KNA0