Detailed information of TA system TA196838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 744146..745899
• Replicon: chromosome
• Accession: NZ_CP071747
• Organism: Burkholderia pseudomallei strain VBM21822

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q63JY0
• Locus tag: J3B45_RS21150
• Protein ID: WP_004188815.1
• Coordinates: 744547..745899 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J3B45_RS21145
• Protein ID: WP_004188446.1
• Coordinates: 744146..744550 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3B45_RS21135 (J3B45_21135)	740684..743080	-	2397	WP_080364570.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
J3B45_RS21140 (J3B45_21140)	743545..743808	+	264	WP_004199789.1	hypothetical protein	-
J3B45_RS21145 (J3B45_21145)	744146..744550	+	405	WP_004188446.1	helix-turn-helix transcriptional regulator	Antitoxin
J3B45_RS21150 (J3B45_21150)	744547..745899	+	1353	WP_004188815.1	type II toxin-antitoxin system HipA family toxin	Toxin
J3B45_RS21155 (J3B45_21155)	746067..746519	-	453	WP_004198842.1	hypothetical protein	-
J3B45_RS21160 (J3B45_21160)	746620..746766	-	147	WP_004199790.1	hypothetical protein	-
J3B45_RS21165 (J3B45_21165)	746936..747850	-	915	WP_004545706.1	LysR family transcriptional regulator	-
J3B45_RS21170 (J3B45_21170)	748017..748970	+	954	WP_004187785.1	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49848.83 Da
• Isoelectric Point: 9.6807

>T196838 WP_004188815.1 NZ_CP071747:744547-745899 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T196838 NZ_CP095448:c2831112-2831010 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14392.75 Da
• Isoelectric Point: 11.7233

>AT196838 WP_004188446.1 NZ_CP071747:744146-744550 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTVANARAGARRAVPPASAPAPSAAGRPPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT196838 NZ_CP095448:2830972-2831243 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGATTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A3N4FSX2