Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 109800..110560 Replicon plasmid pKGB1a
Accession NZ_CP071736
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag KGB1_RS28040 Protein ID WP_049157791.1
Coordinates 109800..110285 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag KGB1_RS28045 Protein ID WP_211754330.1
Coordinates 110273..110560 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS27995 (KGB1_27975) 105475..105912 + 438 WP_211754291.1 hypothetical protein -
KGB1_RS28000 (KGB1_27980) 105982..106675 + 694 Protein_118 IS6 family transposase -
KGB1_RS28005 (KGB1_27985) 106854..107162 - 309 Protein_119 theronine dehydrogenase -
KGB1_RS28010 (KGB1_27990) 107159..107719 - 561 WP_211754292.1 plasmid SOS inhibition protein A -
KGB1_RS28015 (KGB1_27995) 107716..108151 - 436 Protein_121 conjugation system SOS inhibitor PsiB -
KGB1_RS28020 (KGB1_28000) 108192..108710 - 519 Protein_122 hypothetical protein -
KGB1_RS28025 108711..109057 + 347 Protein_123 cytoplasmic protein -
KGB1_RS28030 (KGB1_28005) 109302..109418 - 117 WP_223176086.1 type I toxin-antitoxin system Hok family toxin -
KGB1_RS28035 109366..109518 - 153 WP_224222653.1 DUF5431 family protein -
KGB1_RS28040 (KGB1_28010) 109800..110285 - 486 WP_049157791.1 GNAT family N-acetyltransferase Toxin
KGB1_RS28045 (KGB1_28015) 110273..110560 - 288 WP_211754330.1 DUF1778 domain-containing protein Antitoxin
KGB1_RS28050 (KGB1_28020) 110965..113160 - 2196 WP_000093087.1 DEAD/DEAH box helicase -
KGB1_RS28055 (KGB1_28025) 113157..114473 - 1317 WP_211754293.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..160574 160574
- flank IS/Tn - - 106379..106675 296


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(25-150)

Antitoxin

Vibrio phage ICP1, Orf50

(13-89)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17795.50 Da        Isoelectric Point: 9.4841

>T196835 WP_049157791.1 NZ_CP071736:c110285-109800 [Klebsiella variicola]
VGRVTAPEPLSTYHQVAEFVSGETVLDDWLKQKGLKNQALGAARTFVVCKKDTQQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQERTLFLKLP
Q

Download         Length: 486 bp

>T196835 NZ_CP095448:c2221980-2221873 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 11030.73 Da        Isoelectric Point: 4.8667

>AT196835 WP_211754330.1 NZ_CP071736:c110560-110273 [Klebsiella variicola]
MLYKRCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQHAEFIDMLDAPVTDD
PVIEKLLARKPQWDV

Download         Length: 288 bp

>AT196835 NZ_CP095448:2222028-2222094 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References