Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 34355..35265 Replicon plasmid pKGB1b
Accession NZ_CP071735
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name - Uniprot ID A0A2N4Z2R0
Locus tag KGB1_RS26585 Protein ID WP_004197882.1
Coordinates 34795..35265 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KGB1_RS26580 Protein ID WP_004181895.1
Coordinates 34355..34798 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS26560 (KGB1_26555) 31420..31635 - 216 Protein_42 IS200/IS605 family transposase -
KGB1_RS26565 (KGB1_26560) 31637..32206 + 570 Protein_43 transposase -
KGB1_RS26570 (KGB1_26565) 32561..32983 + 423 WP_004197884.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KGB1_RS26575 (KGB1_26570) 32983..34254 + 1272 WP_004197886.1 translesion error-prone DNA polymerase V subunit UmuC -
KGB1_RS26580 (KGB1_26575) 34355..34798 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KGB1_RS26585 (KGB1_26580) 34795..35265 + 471 WP_004197882.1 RES family NAD+ phosphorylase Toxin
KGB1_RS26590 (KGB1_26585) 35375..35635 - 261 WP_004026352.1 hypothetical protein -
KGB1_RS26595 (KGB1_26590) 36197..37378 - 1182 WP_004883380.1 IS481 family transposase -
KGB1_RS26600 (KGB1_26595) 37450..37608 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
KGB1_RS26605 (KGB1_26600) 38214..39188 + 975 WP_253616105.1 Hok/Gef family protein -
KGB1_RS26610 (KGB1_26605) 39386..39763 - 378 WP_045289277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..206870 206870
- inside IScluster/Tn - - 31853..37378 5525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17633.92 Da        Isoelectric Point: 4.5152

>T196833 WP_004197882.1 NZ_CP071735:34795-35265 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T196833 NZ_CP095448:1141912-1142247 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT196833 WP_004181895.1 NZ_CP071735:34355-34798 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT196833 NZ_CP095448:1141664-1141912 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z2R0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References