Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5375415..5376040 Replicon chromosome
Accession NZ_CP071734
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KGB1_RS25985 Protein ID WP_008807903.1
Coordinates 5375415..5375798 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KGB1_RS25990 Protein ID WP_004150355.1
Coordinates 5375798..5376040 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS25970 (KGB1_25965) 5372781..5373683 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KGB1_RS25975 (KGB1_25970) 5373680..5374315 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KGB1_RS25980 (KGB1_25975) 5374312..5375241 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KGB1_RS25985 (KGB1_25980) 5375415..5375798 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KGB1_RS25990 (KGB1_25985) 5375798..5376040 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KGB1_RS25995 (KGB1_25990) 5376245..5377162 + 918 WP_064166397.1 alpha/beta hydrolase -
KGB1_RS26000 (KGB1_25995) 5377177..5378118 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KGB1_RS26005 (KGB1_26000) 5378163..5378600 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
KGB1_RS26010 (KGB1_26005) 5378597..5379457 - 861 WP_008807907.1 virulence factor BrkB family protein -
KGB1_RS26015 (KGB1_26010) 5379451..5380050 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T196832 WP_008807903.1 NZ_CP071734:c5375798-5375415 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T196832 NZ_CP095448:1040212-1040619 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196832 WP_004150355.1 NZ_CP071734:c5376040-5375798 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196832 NZ_CP095448:1039965-1040231 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References