Detailed information of TA system TA196831

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4827783..4828436
• Replicon: chromosome
• Accession: NZ_CP071734
• Organism: Klebsiella variicola strain KGB_1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KGB1_RS23410
• Protein ID: WP_117134709.1
• Coordinates: 4827783..4828103 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KGB1_RS23415
• Protein ID: WP_117134710.1
• Coordinates: 4828140..4828436 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGB1_RS23390 (KGB1_23395)	4823736..4824374	-	639	WP_117134706.1	HNH endonuclease	-
KGB1_RS23395 (KGB1_23400)	4824554..4825423	+	870	WP_117134707.1	HNH endonuclease	-
KGB1_RS23400 (KGB1_23405)	4825755..4826762	-	1008	WP_047041139.1	restriction endonuclease	-
KGB1_RS23405 (KGB1_23410)	4826839..4827669	-	831	WP_117134708.1	DUF4942 domain-containing protein	-
KGB1_RS23410 (KGB1_23415)	4827783..4828103	-	321	WP_117134709.1	TA system toxin CbtA family protein	Toxin
KGB1_RS23415 (KGB1_23420)	4828140..4828436	-	297	WP_117134710.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KGB1_RS23420 (KGB1_23425)	4828445..4828915	-	471	WP_117134711.1	DNA repair protein RadC	-
KGB1_RS23425 (KGB1_23430)	4828987..4829808	-	822	WP_142132151.1	DUF932 domain-containing protein	-
KGB1_RS23430 (KGB1_23435)	4829907..4830119	-	213	WP_117134713.1	hypothetical protein	-
KGB1_RS23435 (KGB1_23440)	4830201..4830560	-	360	WP_117134714.1	hypothetical protein	-
KGB1_RS23440 (KGB1_23445)	4830647..4831111	-	465	WP_117134715.1	hypothetical protein	-
KGB1_RS23445 (KGB1_23450)	4831166..4831843	-	678	WP_117134716.1	WYL domain-containing protein	-
KGB1_RS23450 (KGB1_23455)	4831903..4832631	-	729	WP_117134717.1	DUF3944 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4827783..4848742	20959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (12-105)

Antitoxin

• Antitoxin CbeA (6-97)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11985.64 Da
• Isoelectric Point: 6.6419

>T196831 WP_117134709.1 NZ_CP071734:c4828103-4827783 [Klebsiella variicola]
MHISSVPATVPVSSRLSPVQVWKQLLKYLLEHHYGLALNDTPFHDDAAIQEHIEAGITLADAVNFLVERYELVRTDRKGF
TWQDQTAFLTATDILRARRATGLINT

Nucleotide

• Length: 321 bp

>T196831 NZ_CP095448:c875765-875388 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10905.56 Da
• Isoelectric Point: 7.8812

>AT196831 WP_117134710.1 NZ_CP071734:c4828436-4828140 [Klebsiella variicola]
IHVINTIPRFGLRLVQEGNRLHYLADRSGLTGGFTPEQLQKLDQAFPQLIGQLEAMLRSGELNPQQQHKVTIQLTGLVCI
ADTRGSCGYVYISIYPTP

Nucleotide

• Length: 297 bp

>AT196831 NZ_CP095448:c876223-875855 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA