Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4187551..4188170 Replicon chromosome
Accession NZ_CP071734
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KGB1_RS20385 Protein ID WP_002892050.1
Coordinates 4187952..4188170 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KGB1_RS20380 Protein ID WP_008805436.1
Coordinates 4187551..4187925 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS20370 (KGB1_20375) 4182706..4183899 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGB1_RS20375 (KGB1_20380) 4183922..4187068 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
KGB1_RS20380 (KGB1_20385) 4187551..4187925 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KGB1_RS20385 (KGB1_20390) 4187952..4188170 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KGB1_RS20390 (KGB1_20395) 4188329..4188895 + 567 WP_023297139.1 maltose O-acetyltransferase -
KGB1_RS20395 (KGB1_20400) 4188867..4188995 - 129 Protein_4007 hypothetical protein -
KGB1_RS20400 (KGB1_20405) 4189032..4189502 + 471 WP_008805434.1 YlaC family protein -
KGB1_RS20405 (KGB1_20410) 4189471..4190928 - 1458 WP_117134547.1 PLP-dependent aminotransferase family protein -
KGB1_RS20410 (KGB1_20415) 4191029..4191727 + 699 WP_023339664.1 GNAT family protein -
KGB1_RS20415 (KGB1_20420) 4191724..4191864 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KGB1_RS20420 (KGB1_20425) 4191864..4192127 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196830 WP_002892050.1 NZ_CP071734:4187952-4188170 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T196830 NZ_CP095448:c655298-654834 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT196830 WP_008805436.1 NZ_CP071734:4187551-4187925 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT196830 NZ_CP095448:c655633-655298 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References