Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2691942..2693530 Replicon chromosome
Accession NZ_CP071734
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag KGB1_RS13270 Protein ID WP_008804919.1
Coordinates 2691942..2693264 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag KGB1_RS13275 Protein ID WP_008804920.1
Coordinates 2693264..2693530 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS13255 (KGB1_13240) 2687007..2689508 + 2502 WP_117134430.1 fimbria/pilus outer membrane usher protein -
KGB1_RS13260 (KGB1_13245) 2689496..2690500 + 1005 WP_039103406.1 fimbrial protein -
KGB1_RS13265 (KGB1_13250) 2690633..2691925 + 1293 WP_253615989.1 glycoside hydrolase family 10 protein -
KGB1_RS13270 (KGB1_13255) 2691942..2693264 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
KGB1_RS13275 (KGB1_13260) 2693264..2693530 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
KGB1_RS13280 (KGB1_13265) 2693796..2694122 - 327 WP_008804921.1 hydroxyisourate hydrolase -
KGB1_RS13285 (KGB1_13270) 2694119..2694619 - 501 WP_008804922.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
KGB1_RS13290 (KGB1_13275) 2694597..2695976 - 1380 WP_008804923.1 nucleobase:cation symporter-2 family protein -
KGB1_RS13295 (KGB1_13280) 2696322..2697476 + 1155 WP_008804924.1 FAD-dependent urate hydroxylase HpxO -
KGB1_RS13300 (KGB1_13285) 2697457..2698380 - 924 WP_008804925.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T196829 WP_008804919.1 NZ_CP071734:c2693264-2691942 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T196829 NZ_CP095448:237867-237974 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTCGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT196829 WP_008804920.1 NZ_CP071734:c2693530-2693264 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT196829 NZ_CP095448:c237810-237755 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References