Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 817313..817970 Replicon chromosome
Accession NZ_CP071734
Organism Klebsiella variicola strain KGB_1

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag KGB1_RS04075 Protein ID WP_002916310.1
Coordinates 817560..817970 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag KGB1_RS04070 Protein ID WP_002916312.1
Coordinates 817313..817579 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGB1_RS04045 (KGB1_04045) 812462..813895 - 1434 WP_117134834.1 6-phospho-beta-glucosidase BglA -
KGB1_RS04050 (KGB1_04050) 814015..814743 - 729 WP_012967245.1 MurR/RpiR family transcriptional regulator -
KGB1_RS04055 (KGB1_04055) 814794..815105 + 312 WP_008806430.1 N(4)-acetylcytidine aminohydrolase -
KGB1_RS04060 (KGB1_04060) 815269..815928 + 660 WP_008806429.1 hemolysin III family protein -
KGB1_RS04065 (KGB1_04065) 816084..817067 - 984 WP_072124459.1 tRNA-modifying protein YgfZ -
KGB1_RS04070 (KGB1_04070) 817313..817579 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
KGB1_RS04075 (KGB1_04075) 817560..817970 + 411 WP_002916310.1 protein YgfX Toxin
KGB1_RS04080 (KGB1_04080) 817977..818498 - 522 WP_008806427.1 flavodoxin FldB -
KGB1_RS04085 (KGB1_04085) 818599..819495 + 897 WP_008806426.1 site-specific tyrosine recombinase XerD -
KGB1_RS04090 (KGB1_04090) 819518..820231 + 714 WP_012540593.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KGB1_RS04095 (KGB1_04095) 820237..821970 + 1734 WP_117106322.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T196819 WP_002916310.1 NZ_CP071734:817560-817970 [Klebsiella variicola]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T196819 NZ_CP095429:60632-60943 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT196819 WP_002916312.1 NZ_CP071734:817313-817579 [Klebsiella variicola]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196819 NZ_CP095429:60940-61359 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References