Detailed information of TA system TA196814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 554947..555530
• Replicon: chromosome
• Accession: NZ_CP071733
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7010

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1S2VZ23
• Locus tag: BLI010_RS02620
• Protein ID: WP_032745284.1
• Coordinates: 554947..555249 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BLI010_RS02625
• Protein ID: WP_206648302.1
• Coordinates: 555246..555530 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI010_RS02605 (BLI010_02605)	550686..553805	-	3120	WP_206648304.1	alpha-mannosidase	-
BLI010_RS02610 (BLI010_02610)	553966..554454	-	489	WP_206648303.1	D-aminoacyl-tRNA deacylase	-
BLI010_RS02615 (BLI010_02615)	554475..554909	+	435	WP_234928031.1	DUF1922 domain-containing protein	-
BLI010_RS02620 (BLI010_02620)	554947..555249	+	303	WP_032745284.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BLI010_RS02625 (BLI010_02625)	555246..555530	+	285	WP_206648302.1	putative addiction module antidote protein	Antitoxin
BLI010_RS02635 (BLI010_02635)	555797..556129	+	333	WP_206648301.1	hypothetical protein	-
BLI010_RS02640 (BLI010_02640)	556204..556896	-	693	WP_206648300.1	hypothetical protein	-
BLI010_RS02645 (BLI010_02645)	556850..557878	-	1029	WP_206648299.1	YcaO-like family protein	-
BLI010_RS02650 (BLI010_02650)	558502..559233	-	732	WP_206648298.1	ABC transporter permease	-
BLI010_RS02655 (BLI010_02655)	559230..560138	-	909	WP_206648297.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (9-92)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11350.08 Da
• Isoelectric Point: 10.4626

>T196814 WP_032745284.1 NZ_CP071733:554947-555249 [Bifidobacterium longum subsp. infantis]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Nucleotide

• Length: 303 bp

>T196814 NZ_CP095428:4172519-4172887 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10132.34 Da
• Isoelectric Point: 4.5368

>AT196814 WP_206648302.1 NZ_CP071733:555246-555530 [Bifidobacterium longum subsp. infantis]
MSTEFTDYDTSEHLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Nucleotide

• Length: 285 bp

>AT196814 NZ_CP095428:4172301-4172522 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A1S2VZ23