Detailed information of TA system TA196813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 467020..467873
• Replicon: chromosome
• Accession: NZ_CP071733
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BLI010_RS02270
• Protein ID: WP_206648341.1
• Coordinates: 467020..467511 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q8G4I4
• Locus tag: BLI010_RS02275
• Protein ID: WP_011068030.1
• Coordinates: 467511..467873 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI010_RS02250 (BLI010_02250)	462758..463660	-	903	WP_032745207.1	Bax inhibitor-1/YccA family protein	-
BLI010_RS02255 (BLI010_02255)	463944..464084	+	141	Protein_443	LuxR C-terminal-related transcriptional regulator	-
BLI010_RS02260 (BLI010_02260)	464125..464766	+	642	WP_206648343.1	GNAT family N-acetyltransferase	-
BLI010_RS02265 (BLI010_02265)	464855..466960	+	2106	WP_206648342.1	DUF262 domain-containing protein	-
BLI010_RS02270 (BLI010_02270)	467020..467511	-	492	WP_206648341.1	PIN domain-containing protein	Toxin
BLI010_RS02275 (BLI010_02275)	467511..467873	-	363	WP_011068030.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLI010_RS02280 (BLI010_02280)	468019..470718	-	2700	WP_207618091.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	368363..488035	119672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-141)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-53)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18343.93 Da
• Isoelectric Point: 4.6408

>T196813 WP_206648341.1 NZ_CP071733:c467511-467020 [Bifidobacterium longum subsp. infantis]
MTDVSMQRILLDTNVLLDYLLHRDDHAKMAEAVMELGAKNNVTLLCASLSLKDIAYLSSSAIRREFKPNESEVENFTRSF
LSSRVPWRCIEQVKEMCDIVAVDESTCDKAFSLQKRHRDFEDNLIIVAAQQSGANCVVTSDAELISHFPEYCKTPAEIVA
ALE

Nucleotide

• Length: 492 bp

>T196813 NZ_CP095428:c3765797-3765414 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 14004.05 Da
• Isoelectric Point: 5.0733

>AT196813 WP_011068030.1 NZ_CP071733:c467873-467511 [Bifidobacterium longum subsp. infantis]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDKMYDEQLDTYYNMKRAD

Nucleotide

• Length: 363 bp

>AT196813 NZ_CP095428:c3766039-3765797 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A1S2W1N3