Detailed information of TA system TA196811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 87355..87994
• Replicon: chromosome
• Accession: NZ_CP071733
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7010

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A4R0T054
• Locus tag: BLI010_RS00415
• Protein ID: WP_065436064.1
• Coordinates: 87355..87711 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BLI010_RS00420
• Protein ID: WP_230578351.1
• Coordinates: 87698..87994 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI010_RS00385 (BLI010_00385)	82621..83613	+	993	WP_206648492.1	ABC transporter ATP-binding protein	-
BLI010_RS00390 (BLI010_00390)	83812..84438	+	627	WP_007052130.1	30S ribosomal protein S4	-
BLI010_RS00395 (BLI010_00395)	84618..85400	-	783	WP_206648491.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
BLI010_RS00400 (BLI010_00400)	85538..86023	-	486	WP_014483808.1	cytidine deaminase	-
BLI010_RS00405 (BLI010_00405)	86114..86470	+	357	WP_025299775.1	SdpI family protein	-
BLI010_RS00410 (BLI010_00410)	86575..87177	+	603	WP_003829233.1	transglutaminase family protein	-
BLI010_RS00415 (BLI010_00415)	87355..87711	-	357	WP_065436064.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLI010_RS00420 (BLI010_00420)	87698..87994	-	297	WP_230578351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLI010_RS00425 (BLI010_00425)	88065..88319	-	255	WP_234782638.1	hypothetical protein	-
BLI010_RS00430 (BLI010_00430)	88583..89257	+	675	WP_206648490.1	histidine phosphatase family protein	-
BLI010_RS00435 (BLI010_00435)	89402..89800	+	399	WP_007052133.1	DUF948 domain-containing protein	-
BLI010_RS00440 (BLI010_00440)	89800..90036	+	237	WP_007053386.1	hypothetical protein	-
BLI010_RS00445 (BLI010_00445)	90165..92843	+	2679	WP_193641406.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13231.08 Da
• Isoelectric Point: 6.3014

>T196811 WP_065436064.1 NZ_CP071733:c87711-87355 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLSLDDGPTGRLSAYDAGSVAWEINELYPGLLA

Nucleotide

• Length: 357 bp

>T196811 NZ_CP095428:c3285470-3285186 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10967.47 Da
• Isoelectric Point: 4.2896

>AT196811 WP_230578351.1 NZ_CP071733:c87994-87698 [Bifidobacterium longum subsp. infantis]
MMAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT196811 NZ_CP095428:c3285702-3285460 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A4R0T054