Detailed information of TA system TA196810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 35342..35897
• Replicon: chromosome
• Accession: NZ_CP071733
• Organism: Bifidobacterium longum subsp. infantis strain JCM 7010

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BLI010_RS00150
• Protein ID: WP_206648514.1
• Coordinates: 35342..35665 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D6ZTS2
• Locus tag: BLI010_RS00155
• Protein ID: WP_013140588.1
• Coordinates: 35652..35897 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLI010_RS00125 (BLI010_00125)	30367..30665	-	299	Protein_21	IS256 family transposase	-
BLI010_RS00130 (BLI010_00130)	31135..32400	-	1266	WP_206648515.1	Fic family protein	-
BLI010_RS00135 (BLI010_00135)	32944..33309	-	366	WP_013140585.1	YccF domain-containing protein	-
BLI010_RS00140 (BLI010_00140)	33547..34416	+	870	WP_003829104.1	class I SAM-dependent methyltransferase	-
BLI010_RS00145 (BLI010_00145)	34511..35125	+	615	WP_003829107.1	cupin domain-containing protein	-
BLI010_RS00150 (BLI010_00150)	35342..35665	-	324	WP_206648514.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BLI010_RS00155 (BLI010_00155)	35652..35897	-	246	WP_013140588.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLI010_RS00160 (BLI010_00160)	36069..37325	+	1257	WP_019728042.1	HipA domain-containing protein	-
BLI010_RS00165 (BLI010_00165)	37746..39017	-	1272	WP_206648513.1	MFS transporter	-
BLI010_RS00170 (BLI010_00170)	39174..39860	-	687	WP_234923874.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11831.61 Da
• Isoelectric Point: 4.7434

>T196810 WP_206648514.1 NZ_CP071733:c35665-35342 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPQPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T196810 NZ_CP095428:c3183076-3182543 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9293.39 Da
• Isoelectric Point: 4.7719

>AT196810 WP_013140588.1 NZ_CP071733:c35897-35652 [Bifidobacterium longum subsp. infantis]
MSIQIATRVSDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT196810 NZ_CP095428:c3183353-3183087 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A087BFX1