Detailed information of TA system TA196809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 6252538..6253759
• Replicon: chromosome
• Accession: NZ_CP071731
• Organism: Pseudomonas aeruginosa strain LYSZa2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LYSZa2_RS28910
• Protein ID: WP_003102740.1
• Coordinates: 6253181..6253759 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LYSZa2_RS28905
• Protein ID: WP_003088112.1
• Coordinates: 6252538..6253092 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYSZa2_RS28880 (LYSZa2_28880)	6248106..6248657	+	552	WP_039700662.1	XRE family transcriptional regulator	-
LYSZa2_RS28885 (LYSZa2_28885)	6248654..6249052	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LYSZa2_RS28890 (LYSZa2_28890)	6249065..6249388	-	324	WP_023875494.1	multidrug efflux SMR transporter	-
LYSZa2_RS28895 (LYSZa2_28895)	6249719..6250180	+	462	WP_077144623.1	(2Fe-2S)-binding protein	-
LYSZa2_RS28900 (LYSZa2_28900)	6250185..6252380	+	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LYSZa2_RS28905 (LYSZa2_28905)	6252538..6253092	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LYSZa2_RS28910 (LYSZa2_28910)	6253181..6253759	+	579	WP_003102740.1	HD domain-containing protein	Toxin
LYSZa2_RS28915 (LYSZa2_28915)	6253868..6255055	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LYSZa2_RS28920 (LYSZa2_28920)	6255045..6257216	-	2172	WP_023116610.1	type I secretion system permease/ATPase	-
LYSZa2_RS28925 (LYSZa2_28925)	6257206..6258483	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T196809 WP_003102740.1 NZ_CP071731:6253181-6253759 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T196809 NZ_CP095428:2568975-2569193 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT196809 WP_003088112.1 NZ_CP071731:6252538-6253092 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT196809 NZ_CP095428:2568574-2568948 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0C7CS07