Detailed information of TA system TA196801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 4506485..4507993
• Replicon: chromosome
• Accession: NZ_CP071730
• Organism: Pseudomonas aeruginosa strain LYSZa5

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX1
• Locus tag: LYSZa5_RS20910
• Protein ID: WP_003119682.1
• Coordinates: 4506485..4507072 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LYSZa5_RS20915
• Protein ID: WP_031640528.1
• Coordinates: 4507139..4507993 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYSZa5_RS20905 (LYSZa5_20905)	4502740..4506219	-	3480	WP_003117605.1	PAS domain-containing hybrid sensor histidine kinase/response regulator	-
LYSZa5_RS20910 (LYSZa5_20910)	4506485..4507072	+	588	WP_003119682.1	GNAT family protein	Toxin
LYSZa5_RS20915 (LYSZa5_20915)	4507139..4507993	+	855	WP_031640528.1	AraC family transcriptional regulator	Antitoxin
LYSZa5_RS20920 (LYSZa5_20920)	4508090..4510255	+	2166	WP_031640529.1	TonB-dependent receptor family protein	-
LYSZa5_RS20925 (LYSZa5_20925)	4510430..4512304	+	1875	WP_003117607.1	MFS transporter	-
LYSZa5_RS20930 (LYSZa5_20930)	4512365..4512574	-	210	WP_003115568.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21901.23 Da
• Isoelectric Point: 9.8676

>T196801 WP_003119682.1 NZ_CP071730:4506485-4507072 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T196801 NZ_CP095426:5011511-5011798 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31810.09 Da
• Isoelectric Point: 7.3168

>AT196801 WP_031640528.1 NZ_CP071730:4507139-4507993 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACACSSLTTYE

Nucleotide

• Length: 855 bp

>AT196801 NZ_CP095426:5011272-5011511 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 1YRE
• PDB: 8GXJ