Detailed information of TA system TA196798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1297859..1298445
• Replicon: chromosome
• Accession: NZ_CP071730
• Organism: Pseudomonas aeruginosa strain LYSZa5

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: LYSZa5_RS06140
• Protein ID: WP_003120987.1
• Coordinates: 1298146..1298445 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: LYSZa5_RS06135
• Protein ID: WP_003448662.1
• Coordinates: 1297859..1298149 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYSZa5_RS06115 (LYSZa5_06115)	1293010..1293219	+	210	WP_003105733.1	cold-shock protein	-
LYSZa5_RS06120 (LYSZa5_06120)	1293441..1295330	+	1890	WP_016851610.1	hypothetical protein	-
LYSZa5_RS06125 (LYSZa5_06125)	1295327..1297303	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
LYSZa5_RS06130 (LYSZa5_06130)	1297444..1297788	+	345	WP_016851612.1	hypothetical protein	-
LYSZa5_RS06135 (LYSZa5_06135)	1297859..1298149	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
LYSZa5_RS06140 (LYSZa5_06140)	1298146..1298445	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LYSZa5_RS06145 (LYSZa5_06145)	1298647..1299768	+	1122	WP_021263359.1	TcpQ domain-containing protein	-
LYSZa5_RS06150 (LYSZa5_06150)	1299768..1301477	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
LYSZa5_RS06155 (LYSZa5_06155)	1301481..1302806	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
LYSZa5_RS06160 (LYSZa5_06160)	1302796..1303329	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1270880..1413042	142162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T196798 WP_003120987.1 NZ_CP071730:c1298445-1298146 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T196798 NZ_CP095426:4174228-4174446 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT196798 WP_003448662.1 NZ_CP071730:c1298149-1297859 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT196798 NZ_CP095426:4173827-4174201 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: G8CP73