Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 87126..88749 Replicon plasmid pL1
Accession NZ_CP071712
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag JQM62_RS27950 Protein ID WP_000088046.1
Coordinates 87424..88749 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag JQM62_RS27945 Protein ID WP_024136327.1
Coordinates 87126..87419 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS27920 (JQM62_27675) 82998..84239 + 1242 WP_001053338.1 VWA domain-containing protein -
JQM62_RS30135 84330..84461 + 132 WP_000374059.1 hypothetical protein -
JQM62_RS27925 (JQM62_27680) 84802..85083 - 282 WP_000077926.1 hypothetical protein -
JQM62_RS27930 (JQM62_27685) 85133..85324 - 192 WP_000398480.1 hypothetical protein -
JQM62_RS27935 (JQM62_27690) 85416..85757 - 342 WP_001151575.1 hypothetical protein -
JQM62_RS27940 (JQM62_27695) 86091..86522 + 432 WP_011152969.1 hypothetical protein -
JQM62_RS27945 (JQM62_27700) 87126..87419 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
JQM62_RS27950 (JQM62_27705) 87424..88749 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
JQM62_RS27955 (JQM62_27710) 88810..89016 + 207 WP_000134171.1 hypothetical protein -
JQM62_RS27960 (JQM62_27715) 89118..89528 + 411 WP_000985911.1 hypothetical protein -
JQM62_RS27965 (JQM62_27720) 89541..90356 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
JQM62_RS27970 (JQM62_27725) 90610..91035 + 426 WP_001043843.1 hypothetical protein -
JQM62_RS27975 (JQM62_27730) 91584..91892 + 309 WP_001224687.1 hypothetical protein -
JQM62_RS27980 (JQM62_27735) 91908..92765 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
JQM62_RS27985 (JQM62_27740) 92827..93030 + 204 WP_001194555.1 hypothetical protein -
JQM62_RS27990 (JQM62_27745) 93372..93728 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / blaTEM-1B / blaLAP-2 / aac(3)-IId / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / floR / blaCTX-M-55 / qnrS1 / mph(A) / ARR-3 / dfrA14 - 1..268215 268215


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T196782 WP_000088046.1 NZ_CP071712:87424-88749 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T196782 NZ_CP095421:4226228-4226446 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT196782 WP_024136327.1 NZ_CP071712:87126-87419 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT196782 NZ_CP095421:4225827-4226201 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References