Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3840820..3841474 Replicon chromosome
Accession NZ_CP071711
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag JQM62_RS18975 Protein ID WP_000244772.1
Coordinates 3841067..3841474 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JQM62_RS18970 Protein ID WP_000354046.1
Coordinates 3840820..3841086 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS18950 (3837107) 3837107..3837418 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
JQM62_RS18955 (3837582) 3837582..3838241 + 660 WP_000250274.1 hemolysin III family protein -
JQM62_RS18960 (3838364) 3838364..3839344 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
JQM62_RS18965 (3839597) 3839597..3840577 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JQM62_RS18970 (3840820) 3840820..3841086 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JQM62_RS18975 (3841067) 3841067..3841474 + 408 WP_000244772.1 protein YgfX Toxin
JQM62_RS18980 (3841514) 3841514..3842035 - 522 WP_001055874.1 flavodoxin FldB -
JQM62_RS18985 (3842147) 3842147..3843043 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JQM62_RS18990 (3843068) 3843068..3843778 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JQM62_RS18995 (3843784) 3843784..3845517 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3838364..3839344 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T196769 WP_000244772.1 NZ_CP071711:3841067-3841474 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T196769 NZ_CP095409:2607-2927 [Acinetobacter pittii]
ATGCTATTCATTGAAACAAGTATTTTTACCAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGAGACTTAATCAAGAATGGCGGAGGCATTCGTAAAGTACGTTGTGCTCAAGGTA
ACAAGGGTAAAAGTGGCGGTATCCGTGTGATCTACTATTGGGTCACAGAAGATGATCAGATCTTTTTCCTGATAGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT196769 WP_000354046.1 NZ_CP071711:3840820-3841086 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196769 NZ_CP095409:2920-3192 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCGATTAAAGAAGCTGGTGCCATCAAACGTAATGAGCTGAAAGCAAG
TCGAGTGACAGAGCTTGAATTGCCAGATATTAAAGAAGTACGTGAGAAAACTGGCTTAACCCAGGCAGAGTTTGCTGCAC
GTTTGCATATCAGCGCAAGAACCCTGCAAAATTGGGAACAGGGCCGTCGCTATCCGACTGGTCCTGCAGCGACCTTAATT
CGTATTCTAGATGCTCATCCAACTTTAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References