Detailed information of TA system TA196760

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 2140651..2141426
• Replicon: chromosome
• Accession: NZ_CP071711
• Organism: Escherichia coli strain EC20017429

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7V7JG91
• Locus tag: JQM62_RS10765
• Protein ID: WP_001193488.1
• Coordinates: 2140651..2141022 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: JQM62_RS10770
• Protein ID: WP_001059301.1
• Coordinates: 2141061..2141426 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQM62_RS10740 (2136609)	2136609..2137325	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
JQM62_RS10745 (2137345)	2137345..2138451	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
JQM62_RS10750 (2138516)	2138516..2139496	+	981	WP_000991444.1	sialate O-acetylesterase	-
JQM62_RS10755 (2139504)	2139504..2139608	-	105	Protein_2117	HNH endonuclease	-
JQM62_RS10760 (2139708)	2139708..2139851	+	144	Protein_2118	HNH endonuclease	-
JQM62_RS10765 (2140651)	2140651..2141022	-	372	WP_001193488.1	TA system toxin CbtA family protein	Toxin
JQM62_RS10770 (2141061)	2141061..2141426	-	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JQM62_RS10775 (2141452)	2141452..2141673	-	222	WP_000691983.1	DUF987 domain-containing protein	-
JQM62_RS10780 (2141670)	2141670..2142212	-	543	WP_015740440.1	DNA repair protein RadC	-
JQM62_RS10785 (2142225)	2142225..2142668	-	444	WP_001096016.1	antirestriction protein	-
JQM62_RS10790 (2142699)	2142699..2143520	-	822	WP_001234408.1	DUF932 domain-containing protein	-
JQM62_RS10795 (2143667)	2143667..2144113	-	447	WP_032162830.1	hypothetical protein	-
JQM62_RS10800 (2144185)	2144185..2144637	-	453	WP_000734321.1	hypothetical protein	-
JQM62_RS10805 (2144673)	2144673..2145389	-	717	WP_000174910.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE / fimB	2133477..2148167	14690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13832.94 Da
• Isoelectric Point: 7.2419

>T196760 WP_001193488.1 NZ_CP071711:c2141022-2140651 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T196760 NZ_CP095406:132320-132622 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT196760 WP_001059301.1 NZ_CP071711:c2141426-2141061 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT196760 NZ_CP095406:132058-132327 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Structures

Toxin

• AlphaFold DB: A0A7V7JG91

Antitoxin

• AlphaFold DB: A0A7V7JFU6