Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1582031..1582868 Replicon chromosome
Accession NZ_CP071711
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name itaT Uniprot ID A0A0F5GET7
Locus tag JQM62_RS08185 Protein ID WP_000227783.1
Coordinates 1582326..1582868 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JQM62_RS08180 Protein ID WP_001297137.1
Coordinates 1582031..1582342 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS08155 (1577051) 1577051..1577998 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JQM62_RS08160 (1578020) 1578020..1580011 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JQM62_RS08165 (1580001) 1580001..1580615 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JQM62_RS08170 (1580615) 1580615..1580944 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JQM62_RS08175 (1580956) 1580956..1581846 + 891 WP_000971336.1 heme o synthase -
JQM62_RS08180 (1582031) 1582031..1582342 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JQM62_RS08185 (1582326) 1582326..1582868 + 543 WP_000227783.1 GNAT family N-acetyltransferase Toxin
JQM62_RS08190 (1582924) 1582924..1583859 - 936 WP_001297127.1 tetratricopeptide repeat protein -
JQM62_RS08195 (1584267) 1584267..1585631 + 1365 WP_001000978.1 MFS transporter -
JQM62_RS08200 (1585759) 1585759..1586250 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JQM62_RS08205 (1586418) 1586418..1587329 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19776.96 Da        Isoelectric Point: 8.0330

>T196758 WP_000227783.1 NZ_CP071711:1582326-1582868 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T196758 NZ_CP095388:c17725-17381 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATTTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGCCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGACTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT196758 WP_001297137.1 NZ_CP071711:1582031-1582342 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT196758 NZ_CP095388:c17982-17719 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F5GET7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References