Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1547953..1548571 Replicon chromosome
Accession NZ_CP071711
Organism Escherichia coli strain EC20017429

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JQM62_RS08015 Protein ID WP_001291435.1
Coordinates 1548353..1548571 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JQM62_RS08010 Protein ID WP_000344800.1
Coordinates 1547953..1548327 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JQM62_RS08000 (1543042) 1543042..1544235 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JQM62_RS08005 (1544258) 1544258..1547407 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JQM62_RS08010 (1547953) 1547953..1548327 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JQM62_RS08015 (1548353) 1548353..1548571 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JQM62_RS08020 (1548743) 1548743..1549293 + 551 Protein_1581 maltose O-acetyltransferase -
JQM62_RS08025 (1549409) 1549409..1549879 + 471 WP_000136192.1 YlaC family protein -
JQM62_RS08030 (1550043) 1550043..1551593 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JQM62_RS08035 (1551635) 1551635..1551988 - 354 WP_000878141.1 DUF1428 family protein -
JQM62_RS08045 (1552367) 1552367..1552678 + 312 WP_000409911.1 MGMT family protein -
JQM62_RS08050 (1552709) 1552709..1553281 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196757 WP_001291435.1 NZ_CP071711:1548353-1548571 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196757 NZ_CP095384:2710467-2710727 [Lacticaseibacillus rhamnosus]
ATGATTAAAACCTGGACCGATGATGCTTGGGCGGACTACATGTATTGGCATGATCAAAACGACAAGCGGACAATCAAACG
AATTAATCAACTCATTCAAGCCATTGACCGTGACCCTTATAAAGGTATCGGAAAACCTGAGCCACTTAGATATGCCCTAA
CCGGAAAATGGTCACGTCGGATTGATCAGGAAAATCGCATCATCTACAGCATTGAAAAGGGCCACATTAATATTTTCGCC
TGCCGCACTCACTACAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196757 WP_000344800.1 NZ_CP071711:1547953-1548327 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196757 NZ_CP095384:2710205-2710474 [Lacticaseibacillus rhamnosus]
ATGGAAGCAACGAATTATAGTAATTTCCGCCGCAACCTTAAGCATTATATGAGTCAAGTCAACGAAGACGCTGAACCGCT
ACTCGTTACCGCTAAAGATGATGATGACGATGTGGTGGTCATGAGCAAGCACGATTTTGACGCCATCGAAGAAACCCTGT
ATTTACTCAGCAATCCCAAGCTGATGGCCAAAATCAAACGTGGTGATGCCCAAATTGCCGCTGGAAAGGCTAAACAGCAC
GAGTTGTTAACGGACTTCGATCATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References