Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 375760..376396 Replicon chromosome
Accession NZ_CP071709
Organism Cytobacillus gottheilii strain 1839

Toxin (Protein)


Gene name mazF Uniprot ID A0A7V7UTN4
Locus tag J1899_RS01955 Protein ID WP_019156408.1
Coordinates 376046..376396 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A7V7UTJ4
Locus tag J1899_RS01950 Protein ID WP_066451796.1
Coordinates 375760..376041 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1899_RS01930 (J1899_01930) 371583..372167 - 585 WP_066450658.1 rhomboid family intramembrane serine protease -
J1899_RS01935 (J1899_01935) 372277..372627 + 351 WP_066450655.1 holo-ACP synthase -
J1899_RS01940 (J1899_01940) 372828..373841 + 1014 WP_214477177.1 outer membrane lipoprotein carrier protein LolA -
J1899_RS01945 (J1899_01945) 374439..375602 + 1164 WP_214477179.1 alanine racemase -
J1899_RS01950 (J1899_01950) 375760..376041 + 282 WP_066451796.1 antitoxin endoai Antitoxin
J1899_RS01955 (J1899_01955) 376046..376396 + 351 WP_019156408.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
J1899_RS01960 (J1899_01960) 376778..377611 + 834 WP_214477181.1 RsbT co-antagonist protein RsbRA -
J1899_RS01965 (J1899_01965) 377608..377973 + 366 WP_066450646.1 STAS domain-containing protein -
J1899_RS01970 (J1899_01970) 377976..378377 + 402 WP_214477183.1 anti-sigma regulatory factor -
J1899_RS01975 (J1899_01975) 378389..379399 + 1011 WP_214477187.1 PP2C family protein-serine/threonine phosphatase -
J1899_RS01980 (J1899_01980) 379460..379792 + 333 WP_066450632.1 anti-sigma factor antagonist -
J1899_RS01985 (J1899_01985) 379789..380262 + 474 WP_066450627.1 anti-sigma B factor RsbW -
J1899_RS01990 (J1899_01990) 380240..381034 + 795 WP_066450624.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13034.08 Da        Isoelectric Point: 4.8998

>T196752 WP_019156408.1 NZ_CP071709:376046-376396 [Cytobacillus gottheilii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQISLGLIEF

Download         Length: 351 bp

>T196752 NZ_CP095376:c9288337-9288095 [Amycolatopsis pretoriensis]
ATGAGCTACTCCGAGTCTCGCGCGAAGTACGCCGAGACGCTCGACTCCGTGGTCAACGACCGCGAAGAGGTCGTGATCAC
TCGCGCCGGGCACGAACCGGTCGTGATTGTTTCCCTTGACGACTACCAGTCGCTCAAGGAAACCGCCTATCTGCTTCGCA
GCCCTGAGAACGCGCGACGTTTGATCACGGCAATCGAGCGGCTGGAGTCCGGCGGCGGGACACAGCACGAACTTGTGGAA
TGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10817.30 Da        Isoelectric Point: 5.2204

>AT196752 WP_066451796.1 NZ_CP071709:375760-376041 [Cytobacillus gottheilii]
VSESSTTTEIMVELPQQLLTELDGFVKQENVNRSELIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT196752 NZ_CP095376:c9288098-9287838 [Amycolatopsis pretoriensis]
ATGAAGCTGGTTTGGGACGAGCTCGCTTGGCATGACTACCTCTGGTGGCAGACCGAAGACCGGAAGGTTCTCAAGCGCAT
CAACGCACTGCTCGAGGATGTCCGGCGGAACGGCAACGAGGGTCTCGGCAAACCTGAACCCCTGAAGCACGGCTTCCAGG
GTTACTGGTCACGCCGGATCACAGACGAGCACCGGCTCGTCTACAAGATCGCCGAAGACGAGATTCGCATCGCGGCCTGT
CGCTACCACTACGGGAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7UTN4

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7UTJ4

References