Detailed information of TA system TA196746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2094053..2094768
• Replicon: chromosome
• Accession: NZ_CP071698
• Organism: Actinobacillus pleuropneumoniae strain SC1810

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3K87_RS09900
• Protein ID: WP_005599445.1
• Coordinates: 2094053..2094418 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: J3K87_RS09905
• Protein ID: WP_005599447.1
• Coordinates: 2094481..2094768 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3K87_RS09885 (J3K87_09870)	2089638..2091344	-	1707	WP_237593408.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
J3K87_RS09890 (J3K87_09875)	2091440..2091721	-	282	WP_005609261.1	DNA-directed RNA polymerase subunit omega	-
J3K87_RS09895 (J3K87_09880)	2091848..2093929	-	2082	WP_237593409.1	ATP-dependent DNA helicase RecG	-
J3K87_RS09900 (J3K87_09885)	2094053..2094418	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3K87_RS09905 (J3K87_09890)	2094481..2094768	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J3K87_RS09910 (J3K87_09895)	2094974..2096689	+	1716	WP_039709409.1	proline--tRNA ligase	-
J3K87_RS09915 (J3K87_09900)	2096755..2096943	-	189	WP_005602684.1	hypothetical protein	-
J3K87_RS09920 (J3K87_09905)	2096946..2097527	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
J3K87_RS09925 (J3K87_09910)	2097616..2098572	+	957	WP_012263408.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
J3K87_RS09930 (J3K87_09915)	2098572..2099165	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13956.02 Da
• Isoelectric Point: 9.0549

>T196746 WP_005599445.1 NZ_CP071698:c2094418-2094053 [Actinobacillus pleuropneumoniae]
IDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T196746 NZ_CP095280:3454768-3455118 [Aeromonas hydrophila]
ATGTGGACAGTAAAAACGACCGAGCTGTTTGATGTTTGGTTTGATGTGCAAGATGACGCGACGCAGGAAAAAGTCTTGGC
TGGACTACTCGCCTTGGCGCAGGGTGGCCCATCGATAGGCCGCCCCTTGGTCGATACCATCAAGGGCTCGCGTTTTACAA
ACTTGAAAGAGCTGAGGGTGCAGCATCAGGGAGAGCCGCTACGTGCATTCTTCGCCTTTGACCCGTTGCGACAAGCCATC
GTGCTATGTGCTGGTAACAAGGGCGGGAACGAGAAGCGGTTTTACAAAGAGATGCTGCCGTTGGCAGAGGCACAGTACGC
CAGGCATCTGGCTGAGTTGGAGGAAAAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT196746 WP_005599447.1 NZ_CP071698:c2094768-2094481 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT196746 NZ_CP095280:3455118-3455420 [Aeromonas hydrophila]
ATGGGAAGAACACTAGACGATTTGCTGGCCTCCCGTTCAGAAGAGAGTCGGCAACGCATCCAGGAAATGGCTAACGAGCT
GTTGCTGGAGGTGCGTATACAGGCGTTACGCGAAGAGCTGGAGGTGTCGCAGGTTGAGTTGGCCAAGGCACTCGGTGTCA
GTCAGCCTGCCGTCGTGGCGATGGAGCAACGTGGCAGTGATATCAAGCTATCCACCATGAAACGCTATGTTGAAGCCATG
GGCGGCAAGCTGCGTGTGGATGTCGAGTTGCCAACCGGACGACACATCGGATTCAACGTCTGA

Structures

Antitoxin

• AlphaFold DB: A3N3C5