Detailed information of TA system TA196743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1588195..1588840
• Replicon: chromosome
• Accession: NZ_CP071698
• Organism: Actinobacillus pleuropneumoniae strain SC1810

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: B0BQU7
• Locus tag: J3K87_RS07315
• Protein ID: WP_005619674.1
• Coordinates: 1588469..1588840 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: J3K87_RS07310
• Protein ID: WP_005598477.1
• Coordinates: 1588195..1588488 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3K87_RS07280 (J3K87_07260)	1583527..1584210	-	684	WP_005605188.1	arginine ABC transporter permease ArtM	-
J3K87_RS07285 (J3K87_07265)	1584210..1584881	-	672	WP_237593313.1	arginine ABC transporter permease ArtQ	-
J3K87_RS07290 (J3K87_07270)	1584887..1585606	-	720	WP_005619670.1	transporter substrate-binding domain-containing protein	-
J3K87_RS07295 (J3K87_07275)	1585626..1586360	-	735	WP_005598473.1	arginine ABC transporter ATP-binding protein ArtP	-
J3K87_RS07300 (J3K87_07280)	1586585..1587082	-	498	WP_005601953.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
J3K87_RS07305 (J3K87_07285)	1587155..1588117	+	963	WP_005619672.1	calcium/sodium antiporter	-
J3K87_RS07310 (J3K87_07290)	1588195..1588488	-	294	WP_005598477.1	helix-turn-helix domain-containing protein	Antitoxin
J3K87_RS07315 (J3K87_07295)	1588469..1588840	-	372	WP_005619674.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3K87_RS07320 (J3K87_07300)	1589034..1590785	+	1752	WP_043948002.1	protein-disulfide reductase DsbD	-
J3K87_RS07325 (J3K87_07305)	1590843..1591412	+	570	WP_005605204.1	elongation factor P hydroxylase	-
J3K87_RS07330 (J3K87_07310)	1591456..1592310	-	855	WP_005598486.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (32-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-85)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 15066.57 Da
• Isoelectric Point: 10.4054

>T196743 WP_005619674.1 NZ_CP071698:c1588840-1588469 [Actinobacillus pleuropneumoniae]
MYEIVFYRDKRGREPVKEFLLRLLKERQEGSRERLHKISHHLSILHLHGTRAGENYIKHLEDRIWQLRPVGDCLLFASII
RGKFVLLHYFAKQNYRIPKREIERAKVRLADLQERIKDEPYWF

Nucleotide

• Length: 372 bp

>T196743 NZ_CP095280:2571561-2571812 [Aeromonas hydrophila]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10935.81 Da
• Isoelectric Point: 10.3120

>AT196743 WP_005598477.1 NZ_CP071698:c1588488-1588195 [Actinobacillus pleuropneumoniae]
MSPIGSSWTSFEQEIFDESEIAETQFRIRLILEIIETRQQLGISQRKLEKLSGVKQSMIARVEKGSVNPSLATVLKLLVP
LGKTLQIVPLDKNKTVR

Nucleotide

• Length: 294 bp

>AT196743 NZ_CP095280:2571809-2572063 [Aeromonas hydrophila]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Structures

Toxin

• AlphaFold DB: A3N206