Detailed information of TA system TA196741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 759961..760611
• Replicon: chromosome
• Accession: NZ_CP071698
• Organism: Actinobacillus pleuropneumoniae strain SC1810

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B0BNU3
• Locus tag: J3K87_RS03510
• Protein ID: WP_005614926.1
• Coordinates: 759961..760350 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BNU4
• Locus tag: J3K87_RS03515
• Protein ID: WP_005604117.1
• Coordinates: 760351..760611 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3K87_RS03500 (J3K87_03475)	755201..758476	-	3276	WP_237593579.1	helicase-related protein	-
J3K87_RS03505 (J3K87_03480)	758602..759825	-	1224	WP_012262907.1	ATP-binding protein	-
J3K87_RS03510 (J3K87_03485)	759961..760350	-	390	WP_005614926.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3K87_RS03515 (J3K87_03490)	760351..760611	-	261	WP_005604117.1	hypothetical protein	Antitoxin
J3K87_RS03520 (J3K87_03495)	760957..761793	+	837	WP_012262908.1	DUF6216 family protein	-
J3K87_RS03525 (J3K87_03500)	761825..763846	-	2022	WP_012262909.1	excinuclease ABC subunit UvrB	-
J3K87_RS03530 (J3K87_03505)	764023..764835	+	813	WP_005607533.1	iron uptake system protein EfeO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14837.17 Da
• Isoelectric Point: 5.1295

>T196741 WP_005614926.1 NZ_CP071698:c760350-759961 [Actinobacillus pleuropneumoniae]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK

Nucleotide

• Length: 390 bp

>T196741 NZ_CP095276:c4759129-4758758 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10289.63 Da
• Isoelectric Point: 5.1867

>AT196741 WP_005604117.1 NZ_CP071698:c760611-760351 [Actinobacillus pleuropneumoniae]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF

Nucleotide

• Length: 261 bp

>AT196741 NZ_CP095276:c4759374-4759132 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGGGAAGCGATAGAACTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A3N029

Antitoxin

• AlphaFold DB: A3N030