Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4178843..4179624 Replicon chromosome
Accession NZ_CP071693
Organism Salmonella sp. SAL-045

Toxin (Protein)


Gene name TacT2 Uniprot ID B5R980
Locus tag J1G49_RS20350 Protein ID WP_000626100.1
Coordinates 4178843..4179334 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B5R979
Locus tag J1G49_RS20355 Protein ID WP_001110452.1
Coordinates 4179331..4179624 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1G49_RS20320 4174303..4174650 + 348 WP_000887832.1 divalent cation tolerance protein CutA -
J1G49_RS20325 4174626..4176329 + 1704 WP_000068885.1 protein-disulfide reductase DsbD -
J1G49_RS20330 4176366..4176941 + 576 WP_001188509.1 transcriptional regulator -
J1G49_RS20340 4177212..4177286 - 75 Protein_3975 helix-turn-helix domain-containing protein -
J1G49_RS20345 4177843..4178595 + 753 WP_000842433.1 non-specific acid phosphatase -
J1G49_RS20350 4178843..4179334 - 492 WP_000626100.1 GNAT family N-acetyltransferase Toxin
J1G49_RS20355 4179331..4179624 - 294 WP_001110452.1 DUF1778 domain-containing protein Antitoxin
J1G49_RS20360 4179941..4180162 + 222 WP_001576552.1 hypothetical protein -
J1G49_RS20365 4180428..4181303 + 876 WP_000921676.1 AraC family transcriptional regulator -
J1G49_RS20370 4181300..4181587 + 288 WP_001541332.1 GerE family regulatory protein -
J1G49_RS20375 4181580..4181888 - 309 WP_072095651.1 ATP-binding cassette domain-containing protein -
J1G49_RS20380 4181824..4182159 + 336 WP_000370528.1 hypothetical protein -
J1G49_RS20385 4182672..4183577 - 906 WP_001268200.1 YjiK family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(85-145)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 17645.45 Da        Isoelectric Point: 7.7297

>T196726 WP_000626100.1 NZ_CP071693:c4179334-4178843 [Salmonella sp. SAL-045]
MISTPEPLHAGHILTPFCCGVDSIDNWLKQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV

Download         Length: 492 bp

>T196726 NZ_CP095271:c4956569-4956258 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10977.61 Da        Isoelectric Point: 8.6141

>AT196726 WP_001110452.1 NZ_CP071693:c4179624-4179331 [Salmonella sp. SAL-045]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT196726 NZ_CP095271:c4956257-4955967 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656IQ80

Antitoxin

Source ID Structure
PDB 7AK7
AlphaFold DB A0A5I1DGA4

References