Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 271975..272630 Replicon plasmid p4
Accession NZ_CP071682
Organism Rhizobium ruizarguesonis strain NZLR24

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R9MWU8
Locus tag J3P73_RS35590 Protein ID WP_018068333.1
Coordinates 272211..272630 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7W6UNS6
Locus tag J3P73_RS35585 Protein ID WP_018496487.1
Coordinates 271975..272211 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J3P73_RS35560 266980..267777 + 798 WP_018068326.1 hypothetical protein -
J3P73_RS35565 267887..268165 + 279 WP_018068327.1 hypothetical protein -
J3P73_RS35570 268358..268681 + 324 WP_131589529.1 hypothetical protein -
J3P73_RS35575 268998..270611 + 1614 WP_131612452.1 hypothetical protein -
J3P73_RS35580 270611..271714 + 1104 WP_207580394.1 hypothetical protein -
J3P73_RS35585 271975..272211 + 237 WP_018496487.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
J3P73_RS35590 272211..272630 + 420 WP_018068333.1 type II toxin-antitoxin system VapC family toxin Toxin
J3P73_RS35595 272871..273245 + 375 WP_075225998.1 hypothetical protein -
J3P73_RS35600 273259..274857 - 1599 WP_131614721.1 asparagine synthase C-terminal domain-containing protein -
J3P73_RS35605 274931..275089 - 159 WP_017958612.1 hypothetical protein -
J3P73_RS35610 275400..275966 - 567 WP_024323795.1 GNAT family N-acetyltransferase -
J3P73_RS35615 276535..277230 + 696 WP_018484262.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..643193 643193


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-117)

Antitoxin

SpoVT-AbrB domain

(7-44)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15674.90 Da        Isoelectric Point: 4.7852

>T196647 WP_018068333.1 NZ_CP071682:272211-272630 [Rhizobium ruizarguesonis]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPERELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Download         Length: 420 bp

>T196647 NZ_CP095240:3593205-3593489 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9051.15 Da        Isoelectric Point: 5.6454

>AT196647 WP_018496487.1 NZ_CP071682:271975-272211 [Rhizobium ruizarguesonis]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQDERDKI

Download         Length: 237 bp

>AT196647 NZ_CP095240:3592973-3593215 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R9MWU8

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6UNS6

References