Detailed information of TA system TA196644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 272715..273391
• Replicon: plasmid p1
• Accession: NZ_CP071679
• Organism: Rhizobium ruizarguesonis strain NZLR24

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3P73_RS25820
• Protein ID: WP_027688084.1
• Coordinates: 272966..273391 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4Q8ZXM1
• Locus tag: J3P73_RS25815
• Protein ID: WP_075224380.1
• Coordinates: 272715..272969 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3P73_RS25785	268070..268963	-	894	WP_027688088.1	NmrA family NAD(P)-binding protein	-
J3P73_RS25790	269033..269455	-	423	WP_024321542.1	MarR family winged helix-turn-helix transcriptional regulator	-
J3P73_RS25795	269654..270057	+	404	Protein_250	hypothetical protein	-
J3P73_RS25800	270131..271096	-	966	WP_027688086.1	alpha/beta hydrolase	-
J3P73_RS25805	271193..271879	-	687	WP_027688085.1	glutathione S-transferase	-
J3P73_RS25810	271984..272559	+	576	WP_018480381.1	TetR/AcrR family transcriptional regulator	-
J3P73_RS25815	272715..272969	+	255	WP_075224380.1	plasmid stabilization protein	Antitoxin
J3P73_RS25820	272966..273391	+	426	WP_027688084.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3P73_RS25825	273837..274625	+	789	WP_130687373.1	siderophore-iron reductase FhuF	-
J3P73_RS25830	274721..274903	-	183	WP_042940897.1	hypothetical protein	-
J3P73_RS25835	275808..277685	+	1878	WP_024321535.1	methyl-accepting chemotaxis protein	-
J3P73_RS25840	277882..278271	+	390	WP_027688081.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..760106	760106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15186.42 Da
• Isoelectric Point: 4.8283

>T196644 WP_027688084.1 NZ_CP071679:272966-273391 [Rhizobium ruizarguesonis]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLDDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARAAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLKVINPWYAPT

Nucleotide

• Length: 426 bp

>T196644 NZ_CP095240:2996468-2996953 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9249.43 Da
• Isoelectric Point: 5.6158

>AT196644 WP_075224380.1 NZ_CP071679:272715-272969 [Rhizobium ruizarguesonis]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNDDVDNLQLVRDKTPAEP
MSFE

Nucleotide

• Length: 255 bp

>AT196644 NZ_CP095240:2996178-2996471 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4Q8ZXM1