Detailed information of TA system TA196641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 219088..219770
• Replicon: plasmid p4
• Accession: NZ_CP071636
• Organism: Rhizobium sp. NZLR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3O30_RS31520
• Protein ID: WP_207585758.1
• Coordinates: 219088..219519 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3O30_RS31525
• Protein ID: WP_207585759.1
• Coordinates: 219516..219770 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3O30_RS31500	214761..215720	-	960	WP_207585754.1	N-acetylglucosamine kinase	-
J3O30_RS31505	215717..216511	-	795	WP_207585755.1	DeoR family transcriptional regulator	-
J3O30_RS31510	216673..217989	-	1317	WP_207585756.1	alpha-galactosidase	-
J3O30_RS31515	218092..218958	+	867	WP_207585757.1	helix-turn-helix domain-containing protein	-
J3O30_RS31520	219088..219519	-	432	WP_207585758.1	PIN domain-containing protein	Toxin
J3O30_RS31525	219516..219770	-	255	WP_207585759.1	plasmid stabilization protein	Antitoxin
J3O30_RS31530	219951..220937	-	987	WP_207585760.1	zinc-binding dehydrogenase	-
J3O30_RS31535	221016..221262	-	247	Protein_193	nitroreductase family protein	-
J3O30_RS31540	222257..223441	+	1185	WP_207585761.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..276669	276669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15516.96 Da
• Isoelectric Point: 7.3189

>T196641 WP_207585758.1 NZ_CP071636:c219519-219088 [Rhizobium sp. NZLR1]
MILLDTNVISEPWRSVPDVAVIAWLDAQAIETLFLSTITIAELRFGIASMPPGKRQAILHDRLEREVLLHFVERILPFGV
TTSQFYSDLMARARLSGKAIGKADGYIAATAAAHGLAVATRDTSPFEAAGLKVINPWSSPKSS

Nucleotide

• Length: 432 bp

>T196641 NZ_CP095240:c2151175-2150768 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9157.27 Da
• Isoelectric Point: 5.6278

>AT196641 WP_207585759.1 NZ_CP071636:c219770-219516 [Rhizobium sp. NZLR1]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILEAAVRPSERVKLGSLLAAIGRDAELSDDDVGALQQGRDKAPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT196641 NZ_CP095240:c2151422-2151156 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA