Detailed information of TA system TA196639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 185645..186300
• Replicon: plasmid p3
• Accession: NZ_CP071635
• Organism: Rhizobium sp. NZLR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3O30_RS29935
• Protein ID: WP_077988169.1
• Coordinates: 185645..186064 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7W6UNS6
• Locus tag: J3O30_RS29940
• Protein ID: WP_018496487.1
• Coordinates: 186064..186300 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3O30_RS29910	181005..181700	-	696	WP_077988165.1	protein rhiA	-
J3O30_RS29915	182277..182831	+	555	WP_077988166.1	GNAT family N-acetyltransferase	-
J3O30_RS29920	183180..183338	+	159	WP_165505241.1	hypothetical protein	-
J3O30_RS29925	183411..185009	+	1599	WP_077988167.1	asparagine synthase	-
J3O30_RS29930	185141..185389	-	249	Protein_196	amidase	-
J3O30_RS29935	185645..186064	-	420	WP_077988169.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3O30_RS29940	186064..186300	-	237	WP_018496487.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J3O30_RS29945	186721..187578	-	858	WP_131713911.1	hypothetical protein	-
J3O30_RS29950	187663..188664	-	1002	WP_077988171.1	hypothetical protein	-
J3O30_RS29955	188802..189275	-	474	WP_131624556.1	hypothetical protein	-
J3O30_RS29960	189453..189857	-	405	WP_131624554.1	hypothetical protein	-
J3O30_RS29965	190102..190380	-	279	WP_018068327.1	hypothetical protein	-
J3O30_RS29970	190490..191158	-	669	WP_131624552.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..328074	328074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15660.87 Da
• Isoelectric Point: 4.7852

>T196639 WP_077988169.1 NZ_CP071635:c186064-185645 [Rhizobium sp. NZLR1]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPERELDNLSALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T196639 NZ_CP095240:292706-294028 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9051.15 Da
• Isoelectric Point: 5.6454

>AT196639 WP_018496487.1 NZ_CP071635:c186300-186064 [Rhizobium sp. NZLR1]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQDERDKI

Nucleotide

• Length: 237 bp

>AT196639 NZ_CP095240:292440-292706 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W6UNS6