Detailed information of TA system TA196638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 398005..398549
• Replicon: plasmid p2
• Accession: NZ_CP071634
• Organism: Rhizobium sp. NZLR1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J3O30_RS28485
• Protein ID: WP_207585338.1
• Coordinates: 398226..398549 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J3O30_RS28480
• Protein ID: WP_207585337.1
• Coordinates: 398005..398229 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3O30_RS28465	394097..394378	+	282	WP_207585334.1	sarcosine oxidase subunit delta	-
J3O30_RS28470	394375..397332	+	2958	WP_207585335.1	sarcosine oxidase subunit alpha family protein	-
J3O30_RS28475	397325..397873	+	549	WP_207585336.1	sarcosine oxidase subunit gamma	-
J3O30_RS28480	398005..398229	+	225	WP_207585337.1	DUF3018 family protein	Antitoxin
J3O30_RS28485	398226..398549	+	324	WP_207585338.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J3O30_RS28490	398598..399977	-	1380	Protein_367	CpaF family protein	-
J3O30_RS28495	400380..400583	+	204	WP_007628054.1	hypothetical protein	-
J3O30_RS28500	400597..401160	+	564	WP_207585339.1	prepilin peptidase	-
J3O30_RS28505	401166..401963	+	798	WP_207585340.1	pilus assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..492934	492934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11331.30 Da
• Isoelectric Point: 10.5847

>T196638 WP_207585338.1 NZ_CP071634:398226-398549 [Rhizobium sp. NZLR1]
VRRGDIVTVAITGDFGKPRPALIIQADQFDATGTVTVLPISGTLIDAPLIRPTIEPTALSGLRKASQVKVDKSMSVKRER
IGPIIGRLEDDALLAVTRSLAVFLGIA

Nucleotide

• Length: 324 bp

>T196638 NZ_CP095239:c2794-2528 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8242.15 Da
• Isoelectric Point: 4.7496

>AT196638 WP_207585337.1 NZ_CP071634:398005-398229 [Rhizobium sp. NZLR1]
MATPVNERVQKRREALRAAGLRPVQIWVPDTRRPGFADECHRQSVQTALADAGDRDLQDFLDAALADMDHDGEA

Nucleotide

• Length: 225 bp

>AT196638 NZ_CP095239:c3002-2778 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA