Detailed information of TA system TA196636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 270314..270999
• Replicon: plasmid p1
• Accession: NZ_CP071633
• Organism: Rhizobium sp. NZLR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J3O30_RS23665
• Protein ID: WP_207585005.1
• Coordinates: 270577..270999 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J3O30_RS23660
• Protein ID: WP_207585004.1
• Coordinates: 270314..270580 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3O30_RS23640	266020..266709	+	690	WP_207585001.1	ABC transporter permease subunit	-
J3O30_RS23645	266830..267738	+	909	WP_207585002.1	homocysteine S-methyltransferase family protein	-
J3O30_RS23650	267759..268187	-	429	WP_207585176.1	MarR family transcriptional regulator	-
J3O30_RS23655	268387..270198	+	1812	WP_207585003.1	chloride channel protein	-
J3O30_RS23660	270314..270580	+	267	WP_207585004.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
J3O30_RS23665	270577..270999	+	423	WP_207585005.1	PIN domain-containing protein	Toxin
J3O30_RS23670	271026..271796	-	771	WP_207585006.1	ATP-binding cassette domain-containing protein	-
J3O30_RS23675	271806..272789	-	984	WP_207585007.1	ABC transporter substrate-binding protein	-
J3O30_RS23680	272830..273681	-	852	WP_207585008.1	ABC transporter permease subunit	-
J3O30_RS23685	273694..275046	-	1353	WP_207585009.1	NtaA/DmoA family FMN-dependent monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / htpB	1..913960	913960

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-60)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15630.94 Da
• Isoelectric Point: 5.6327

>T196636 WP_207585005.1 NZ_CP071633:270577-270999 [Rhizobium sp. NZLR1]
VRLLLDTNVLSEVTKPKPDAHVLQWLDRLDEDRSFISIVSVAEIRRGVALMDEGRKRDALAEWLARDLTQRFEHRIIPVD
ESVALAWGDLMGHAKRSGRGLPSMDGLIAATAVAHDLTLATRNSKDFDGLGIELINPWIL

Nucleotide

• Length: 423 bp

>T196636 NZ_CP095236:57980-58129 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9860.03 Da
• Isoelectric Point: 6.3499

>AT196636 WP_207585004.1 NZ_CP071633:270314-270580 [Rhizobium sp. NZLR1]
MQPSRQDNESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARQGTLAEFLMESPLRGANLDLERQR
DEPRDPAL

Nucleotide

• Length: 267 bp

>AT196636 NZ_CP095236:c57935-57876 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT